Human DCTN1 protein (Recombinant) (N-His) (STJP008505)

SKU:
STJP008505
£328.50 - £1,437.50
Free Shipping
Processing The item has been added

Host: E. coli
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description : Recombinant-Human DCTN1-N-His protein was developed from e. coli and has a target region of N-His. For use in research applications.
Formulation: Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Storage Instruction: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt.
Gene Symbol: DCTN1
Gene ID: 1639
Uniprot ID: DCTN1_HUMAN
Immunogen Region: Pro599-Glu911
Immunogen: Homo sapiens (Human)
Post Translational Modifications Ubiquitinated by a SCF complex containing FBXL5, leading to its degradation by the proteasome. Phosphorylation by SLK at Thr-145, Thr-146 and Thr-147 targets DCTN1 to the centrosome. It is uncertain if SLK phosphorylates all three threonines or one or two of them. PLK1-mediated phosphorylation at Ser-179 is essential for its localization in the nuclear envelope, promotes its dissociation from microtubules during early mitosis and positively regulates nuclear envelope breakdown during prophase.
Function Part of the dynactin complex that activates the molecular motor dynein for ultra-processive transport along microtubules. Plays a key role in dynein-mediated retrograde transport of vesicles and organelles along microtubules by recruiting and tethering dynein to microtubules. Binds to both dynein and microtubules providing a link between specific cargos, microtubules and dynein. Essential for targeting dynein to microtubule plus ends, recruiting dynein to membranous cargos and enhancing dynein processivity (the ability to move along a microtubule for a long distance without falling off the track). Can also act as a brake to slow the dynein motor during motility along the microtubule. Can regulate microtubule stability by promoting microtubule formation, nucleation and polymerization and by inhibiting microtubule catastrophe in neurons. Inhibits microtubule catastrophe by binding both to microtubules and to tubulin, leading to enhanced microtubule stability along the axon. Plays a role in metaphase spindle orientation. Plays a role in centriole cohesion and subdistal appendage organization and function. Its recruitment to the centriole in a KIF3A-dependent manner is essential for the maintenance of centriole cohesion and the formation of subdistal appendage. Also required for microtubule anchoring at the mother centriole. Plays a role in primary cilia formation.
Protein Name Dynactin Subunit 1
150 Kda Dynein-Associated Polypeptide
Dap-150
Dp-150
P135
P150-Glued
Database Links Reactome: R-HSA-2132295
Reactome: R-HSA-2565942 Q14203-2
Reactome: R-HSA-3371497
Reactome: R-HSA-380259 Q14203-2
Reactome: R-HSA-380270 Q14203-2
Reactome: R-HSA-380284 Q14203-2
Reactome: R-HSA-380320 Q14203-2
Reactome: R-HSA-381038
Reactome: R-HSA-5620912 Q14203-2
Reactome: R-HSA-6807878
Reactome: R-HSA-6811436
Reactome: R-HSA-8854518 Q14203-2
Reactome: R-HSA-9725370
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Centriole
Spindle
Nucleus Envelope
Cell Cortex
Localizes To Microtubule Plus Ends
Localizes Preferentially To The Ends Of Tyrosinated Microtubules
Localization At Centrosome Is Regulated By Slk-Dependent Phosphorylation
Localizes To Centrosome In A Parkda-Dependent Manner
Localizes To The Subdistal Appendage Region Of The Centriole In A Kif3a-Dependent Manner
Plk1-Mediated Phosphorylation At Ser-179 Is Essential For Its Localization In The Nuclear Envelope
Alternative Protein Names Dynactin Subunit 1 protein
150 Kda Dynein-Associated Polypeptide protein
Dap-150 protein
Dp-150 protein
P135 protein
P150-Glued protein
DCTN1 protein

Information sourced from Uniprot.org