| Post Translational Modifications | Ubiquitinated by a SCF complex containing FBXL5, leading to its degradation by the proteasome. Phosphorylation by SLK at Thr-145, Thr-146 and Thr-147 targets DCTN1 to the centrosome. It is uncertain if SLK phosphorylates all three threonines or one or two of them. PLK1-mediated phosphorylation at Ser-179 is essential for its localization in the nuclear envelope, promotes its dissociation from microtubules during early mitosis and positively regulates nuclear envelope breakdown during prophase. |
| Function | Part of the dynactin complex that activates the molecular motor dynein for ultra-processive transport along microtubules. Plays a key role in dynein-mediated retrograde transport of vesicles and organelles along microtubules by recruiting and tethering dynein to microtubules. Binds to both dynein and microtubules providing a link between specific cargos, microtubules and dynein. Essential for targeting dynein to microtubule plus ends, recruiting dynein to membranous cargos and enhancing dynein processivity (the ability to move along a microtubule for a long distance without falling off the track). Can also act as a brake to slow the dynein motor during motility along the microtubule. Can regulate microtubule stability by promoting microtubule formation, nucleation and polymerization and by inhibiting microtubule catastrophe in neurons. Inhibits microtubule catastrophe by binding both to microtubules and to tubulin, leading to enhanced microtubule stability along the axon. Plays a role in metaphase spindle orientation. Plays a role in centriole cohesion and subdistal appendage organization and function. Its recruitment to the centriole in a KIF3A-dependent manner is essential for the maintenance of centriole cohesion and the formation of subdistal appendage. Also required for microtubule anchoring at the mother centriole. Plays a role in primary cilia formation. |
| Protein Name | Dynactin Subunit 1150 Kda Dynein-Associated PolypeptideDap-150Dp-150P135P150-Glued |
| Database Links | Reactome: R-HSA-2132295Reactome: R-HSA-2565942 Q14203-2Reactome: R-HSA-3371497Reactome: R-HSA-380259 Q14203-2Reactome: R-HSA-380270 Q14203-2Reactome: R-HSA-380284 Q14203-2Reactome: R-HSA-380320 Q14203-2Reactome: R-HSA-381038Reactome: R-HSA-5620912 Q14203-2Reactome: R-HSA-6807878Reactome: R-HSA-6811436Reactome: R-HSA-8854518 Q14203-2Reactome: R-HSA-9725370 |
| Cellular Localisation | CytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeCentrioleSpindleNucleus EnvelopeCell CortexLocalizes To Microtubule Plus EndsLocalizes Preferentially To The Ends Of Tyrosinated MicrotubulesLocalization At Centrosome Is Regulated By Slk-Dependent PhosphorylationLocalizes To Centrosome In A Parkda-Dependent MannerLocalizes To The Subdistal Appendage Region Of The Centriole In A Kif3a-Dependent MannerPlk1-Mediated Phosphorylation At Ser-179 Is Essential For Its Localization In The Nuclear Envelope |
| Alternative Protein Names | Dynactin Subunit 1 protein150 Kda Dynein-Associated Polypeptide proteinDap-150 proteinDp-150 proteinP135 proteinP150-Glued proteinDCTN1 protein |
Information sourced from Uniprot.org
