| Host: | E. coli |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Recombinant-Human ATG5-N-Avi-His protein was developed from e. coli and has a target region of N-Avi-His. For use in research applications. |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction: | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
| Gene Symbol: | ATG5 |
| Gene ID: | 9474 |
| Uniprot ID: | ATG5_HUMAN |
| Immunogen Region: | Met1-Asp275 |
| Immunogen: | Homo sapiens (Human) |
| Post Translational Modifications | Conjugated to ATG12.which is essential for autophagy, but is not required for association with isolation membrane. Acetylated by EP300. |
| Function | Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT20 via the autophagic pathway. As part of the ATG8 conjugation system with ATG12 and ATG16L1, required for recruitment of LRRK2 to stressed lysosomes and induction of LRRK2 kinase activity in response to lysosomal stress. May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Plays a crucial role in IFN-gamma-induced autophagic cell death by interacting with FADD. (Microbial infection) May act as a proviral factor. In association with ATG12, negatively regulates the innate antiviral immune response by impairing the type I IFN production pathway upon vesicular stomatitis virus (VSV) infection. Required for the translation of incoming hepatitis C virus (HCV) RNA and, thereby, for initiation of HCV replication, but not required once infection is established. |
| Protein Name | Autophagy Protein 5Apg5-LikeApoptosis-Specific Protein |
| Database Links | Reactome: R-HSA-1632852Reactome: R-HSA-5205685Reactome: R-HSA-8934903Reactome: R-HSA-936440 |
| Cellular Localisation | CytoplasmPreautophagosomal Structure MembranePeripheral Membrane ProteinColocalizes With Nonmuscle ActinThe Conjugate Detaches From The Membrane Immediately Before Or After Autophagosome Formation Is CompletedLocalizes Also To Discrete Punctae Along The Ciliary Axoneme And To The Base Of The Ciliary Axoneme |
| Alternative Protein Names | Autophagy Protein 5 proteinApg5-Like proteinApoptosis-Specific Protein proteinATG5 proteinAPG5L proteinASP protein |
Information sourced from Uniprot.org
