| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.47ng/mL |
| Detection Limit: |
0.78~50ng/mL |
| Short Description: |
This human AOC1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
AOC1 |
| Gene ID: |
26 |
| Uniprot ID: |
AOC1_HUMAN |
| Specificity: |
This kit recognizes Human DAO in samples. No significant cross-reactivity or interference between Human DAO and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Placenta and kidney. |
| Post Translational Modifications | Topaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue. |
| Function | Catalyzes the degradation of compounds such as putrescine, histamine, spermine, and spermidine, substances involved in allergic and immune responses, cell proliferation, tissue differentiation, tumor formation, and possibly apoptosis. Placental DAO is thought to play a role in the regulation of the female reproductive function. |
| Protein Name | Amiloride-Sensitive Amine Oxidase Copper-ContainingDaoDiamine OxidaseAmiloride-Binding Protein 1Amine Oxidase Copper Domain-Containing Protein 1HistaminaseKidney Amine OxidaseKao |
| Database Links | Reactome: R-HSA-211945Reactome: R-HSA-6798695 |
| Cellular Localisation | SecretedExtracellular Space |
| Alternative CLIA Names | Amiloride-Sensitive Amine Oxidase Copper-Containing CLIA kitDao CLIA kitDiamine Oxidase CLIA kitAmiloride-Binding Protein 1 CLIA kitAmine Oxidase Copper Domain-Containing Protein 1 CLIA kitHistaminase CLIA kitKidney Amine Oxidase CLIA kitKao CLIA kitAOC1 CLIA kitABP1 CLIA kitDAO1 CLIA kit |
| Specificity | This kit recognizes Human DAO in samples. No significant cross-reactivity or interference between Human DAO and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 95-111 | 101 |
| EDTA plasma(n=8) | 89-101 | 96 |
| Cell culture media(n=8) | 96-114 | 104 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 2.49 | 4.70 | 17.20 | 2.32 | 4.23 | 18.26 |
| Standard deviation | 0.23 | 0.52 | 1.09 | 0.26 | 0.31 | 1.16 |
| CV (%) | 9.24 | 11.06 | 6.34 | 11.21 | 7.33 | 6.35 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
