• Western blot analysis of extracts of mouse kidney, using ZCCHC4 antibody (STJ112183) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ZCCHC4 antibody (50-220) (STJ112183)

SKU:
STJ112183

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Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ZCCHC4 (50-220) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ZCCHC4
Gene ID: 29063
Uniprot ID: ZCHC4_HUMAN
Immunogen Region: 50-220
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 50-220 of human ZCCHC4 (NP_079212.2).
Immunogen Sequence: KVTQGKEETRRFYACSACRD RKDCNFFQWEDEKLSGARLA AREAHNRRCQPPLSRTQCVE RYLKFIELPLTQRKFCQTCQ QLLLPDDWGQHSEHQVLGNV SITQLRRPSQLLYPLENKKT NAQYLFADRSCQFLVDLLSA LGFRRVLCVGTPRLHELIKL TASGDKKSNIK
Function rRNA N6-methyltransferase that specifically methylates the adenine in position 4220 of 28S rRNA. N6-methylation of adenine(4220) in 28S rRNA is required for translation.
Protein Name Rrna N6-Adenosine-Methyltransferase Zcchc4
Zinc Finger Cchc Domain-Containing Protein 4
Cellular Localisation Nucleus
Nucleolus
Cytoplasm
Accumulates In The Nucleolus
Where Ribosome Biogenesis Takes Place
Alternative Antibody Names Anti-Rrna N6-Adenosine-Methyltransferase Zcchc4 antibody
Anti-Zinc Finger Cchc Domain-Containing Protein 4 antibody
Anti-ZCCHC4 antibody

Information sourced from Uniprot.org

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