• Immunofluorescence analysis of U-2 OS cells using WDR18 Rabbit polyclonal antibody (STJ118320) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using WDR18 Rabbit polyclonal antibody (STJ118320) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 120s.

Anti-WDR18 antibody (243-432) (STJ118320)

SKU:
STJ118320

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-WDR18 (243-432) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: WDR18
Gene ID: 57418
Uniprot ID: WDR18_HUMAN
Immunogen Region: 243-432
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 243-432 of human WDR18 (NP_077005.2).
Immunogen Sequence: DLFTWPGQRERSFHPEQDAG KVFKGHRNQVTCLSVSTDGS VLLSGSHDETVRLWDVQSKQ CIRTVALKGPVTNAAILLAP VSMLSSDFRPSLPLPHFNKH LLGAEHGDEPRHGGLTLRLG LHQQGSEPSYLDRTEQLQAV LCSTMEKSVLGGQDQLRVRV TELEDEVRNLRKINRDLFDF STRFITRPAK
Function Functions as a component of the Five Friends of Methylated CHTOP (5FMC) complex.the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes. Component of the PELP1 complex involved in the nucleolar steps of 28S rRNA maturation and the subsequent nucleoplasmic transit of the pre-60S ribosomal subunit. May play a role during development.
Protein Name Wd Repeat-Containing Protein 18
Database Links Reactome: R-HSA-6791226
Cellular Localisation Nucleus
Nucleolus
Nucleoplasm
Cytoplasm
Dynein Axonemal Particle
Mainly Found In The Nucleoplasm
With Low Levels Detected In The Cytoplasmic And Chromatin Fractions
Alternative Antibody Names Anti-Wd Repeat-Containing Protein 18 antibody
Anti-WDR18 antibody

Information sourced from Uniprot.org

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