| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 IHC-P:1:50-1:200 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | WASL |
| Gene ID: | 8976 |
| Uniprot ID: | WASL_HUMAN |
| Immunogen Region: | 1-250 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human N-WASP/WASL (NP_003932.3). |
| Immunogen Sequence: | MSSVQQQPPPPRRVTNVGSL LLTPQENESLFTFLGKKCVT MSSAVVQLYAADRNCMWSKK CSGVACLVKDNPQRSYFLRI FDIKDGKLLWEQELYNNFVY NSPRGYFHTFAGDTCQVALN FANEEEAKKFRKAVTDLLGR RQRKSEKRRDPPNGPNLPMA TVDIKNPEITTNRFYGPQVN NISHTKEKKKGKAKKKRLTK ADIGTPSNFQHIGHVGWDPN TGFDLNNLDPELKNLFDMC |
| Post Translational Modifications | Phosphorylation at Ser-242, Tyr-256, Ser-484 and Ser-485 enhances actin polymerization activity. |
| Function | Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Involved in various processes, such as mitosis and cytokinesis, via its role in the regulation of actin polymerization. Together with CDC42, involved in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia. In addition to its role in the cytoplasm, also plays a role in the nucleus by regulating gene transcription, probably by promoting nuclear actin polymerization. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression. Plays a role in dendrite spine morphogenesis. Decreasing levels of DNMBP (using antisense RNA) alters apical junction morphology in cultured enterocytes, junctions curve instead of being nearly linear. |
| Protein Name | Actin Nucleation-Promoting Factor Wasl Neural Wiskott-Aldrich Syndrome Protein N-Wasp |
| Database Links | Reactome: R-HSA-2029482 Reactome: R-HSA-203641 Reactome: R-HSA-373753 Reactome: R-HSA-3928662 Reactome: R-HSA-418885 Reactome: R-HSA-5663213 Reactome: R-HSA-8856828 Reactome: R-HSA-9013148 Reactome: R-HSA-9013149 Reactome: R-HSA-9013406 Reactome: R-HSA-9013409 Reactome: R-HSA-9013424 Reactome: R-HSA-9664422 |
| Cellular Localisation | Cytoplasm Cytoskeleton Nucleus Preferentially Localized In The Cytoplasm When Phosphorylated And In The Nucleus When Unphosphorylated Exported From The Nucleus By An Nuclear Export Signal (Nes)-Dependent Mechanism To The Cytoplasm |
| Alternative Antibody Names | Anti-Actin Nucleation-Promoting Factor Wasl antibody Anti-Neural Wiskott-Aldrich Syndrome Protein antibody Anti-N-Wasp antibody Anti-WASL antibody |
Information sourced from Uniprot.org