Western blot analysis of lysates from mouse spleen, using VAMP5 Rabbit pAb (STJ116583) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-VAMP5 antibody (1-116) (STJ116583)

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STJ116583
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: VAMP5
Gene ID: 10791
Uniprot ID: VAMP5_HUMAN
Immunogen Region: 1-116
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-116 of human VAMP5 (NP_006625.1).
Immunogen Sequence: MAGIELERCQQQANEVTEIM RNNFGKVLERGVKLAELQQR SDQLLDMSSTFNKTTQNLAQ KKCWENIRYRICVGLVVVGV LLIILIVLLVVFLPQSSDSS SAPRTQDAGIASGPGN
Post Translational Modifications (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type X (BoNT/X) which hydrolyzes the 40-Arg-|-Ser-41 bond and probably inhibits neurotransmitter release. It remains unknown whether BoNT/X is ever produced, or what organisms it targets.
Function May participate in trafficking events that are associated with myogenesis, such as myoblast fusion and/or GLUT4 trafficking.
Protein Name Vesicle-Associated Membrane Protein 5
Vamp-5
Myobrevin
Cellular Localisation Cell Membrane
Single-Pass Type Iv Membrane Protein
Endomembrane System
Golgi Apparatus
Trans-Golgi Network Membrane
Associated With The Plasma Membrane As Well As Intracellular Perinuclear And Peripheral Vesicular Structures Of Myotubes
Associated With The Trans-Golgi
But Not With The Cis-Golgi Apparatus
Alternative Antibody Names Anti-Vesicle-Associated Membrane Protein 5 antibody
Anti-Vamp-5 antibody
Anti-Myobrevin antibody
Anti-VAMP5 antibody
Anti-HSPC191 antibody

Information sourced from Uniprot.org

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