• Immunofluorescence analysis of U-2 OS cells using UFC1 Rabbit polyclonal antibody (STJ115158) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using UFC1 Rabbit polyclonal antibody (STJ115158) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using UFC1 Rabbit polyclonal antibody (STJ115158) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using UFC1 Rabbit polyclonal antibody (STJ115158) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-UFC1 antibody (1-167) (STJ115158)

SKU:
STJ115158

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-UFC1 (1-167) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: UFC1
Gene ID: 51506
Uniprot ID: UFC1_HUMAN
Immunogen Region: 1-167
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-167 of human UFC1 (NP_057490.2).
Immunogen Sequence: MADEATRRVVSEIPVLKTNA GPRDRELWVQRLKEEYQSLI RYVENNKNADNDWFRLESNK EGTRWFGKCWYIHDLLKYEF DIEFDIPITYPTTAPEIAVP ELDGKTAKMYRGGKICLTDH FKPLWARNVPKFGLAHLMAL GLGPWLAVEIPDLIQKGVIQ HKEKCNQ
Function E1-like enzyme which specifically catalyzes the second step in ufmylation. Accepts the ubiquitin-like modifier UFM1 from the E1 enzyme UBA5 and forms an intermediate with UFM1 via a thioester linkage. Ufmylation is involved in reticulophagy (also called ER-phagy) induced in response to endoplasmic reticulum stress.
Protein Name Ubiquitin-Fold Modifier-Conjugating Enzyme 1
Ufm1-Conjugating Enzyme 1
Alternative Antibody Names Anti-Ubiquitin-Fold Modifier-Conjugating Enzyme 1 antibody
Anti-Ufm1-Conjugating Enzyme 1 antibody
Anti-UFC1 antibody
Anti-CGI-126 antibody
Anti-HSPC155 antibody

Information sourced from Uniprot.org

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