| Host: |
Rabbit |
| Applications: |
WB/IF/ICC/ELISA |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
UBE2A |
| Gene ID: |
7319 |
| Uniprot ID: |
UBE2A_HUMAN |
| Immunogen Region: |
1-152 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-152 of human UBE2A (NP_003327.2). |
| Immunogen Sequence: |
MSTPARRRLMRDFKRLQEDP PAGVSGAPSENNIMVWNAVI FGPEGTPFEDGTFKLTIEFT EEYPNKPPTVRFVSKMFHPN VYADGSICLDILQNRWSPTY DVSSILTSIQSLLDEPNPNS PANSQAAQLYQENKREYEKR VSAIVEQSWRDC |
| Post Translational Modifications | Phosphorylation at Ser-120 by CDK9 increases activity towards histone H2B. |
| Function | E2 ubiquitin-conjugating enzyme that accepts ubiquitin from the ubiquitin-activating enzyme E1 and transfers it to a E3 ubiquitin-protein ligase. In vitro catalyzes 'Lys-11', as well as 'Lys-48'-linked polyubiquitination. Together with the E3 enzyme BRE1 (RNF20 and/or RNF40), plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. Involved in mitophagy by acting as a E2 ubiquitin-conjugating enzyme for PRKN. In association with the E3 enzyme UBR4, is involved in N-end rule-dependent protein degradation. In association with the E3 ubiquitin-protein ligase complex SIFI, inhibits the mitochondrial stress response by acting as a E2 ubiquitin-conjugating enzyme for UBR4 and KCMF1. |
| Protein Name | Ubiquitin-Conjugating Enzyme E2 AE2 Ubiquitin-Conjugating Enzyme ARad6 Homolog AHr6aHhr6aUbiquitin Carrier Protein AUbiquitin-Protein Ligase A |
| Database Links | Reactome: R-HSA-8866652Reactome: R-HSA-8866654Reactome: R-HSA-983168 |
| Cellular Localisation | Late EndosomeLysosome |
| Alternative Antibody Names | Anti-Ubiquitin-Conjugating Enzyme E2 A antibodyAnti-E2 Ubiquitin-Conjugating Enzyme A antibodyAnti-Rad6 Homolog A antibodyAnti-Hr6a antibodyAnti-Hhr6a antibodyAnti-Ubiquitin Carrier Protein A antibodyAnti-Ubiquitin-Protein Ligase A antibodyAnti-UBE2A antibodyAnti-RAD6A antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
