| Host: | Mouse |
| Applications: | ELISA/FC/WB/IF/IHC |
| Reactivity: | Human/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-TNF-alpha is suitable for use in ELISA, Flow Cytometry, Western Blot, Immunofluorescence and Immunohistochemistry research applications. |
| Clonality : | Monoclonal |
| Clone ID : | EBS-O-174 |
| Conjugation: | Unconjugated |
| Isotype: | IgG1 Kappa |
| Formulation: | PBS with 0.02% Sodium Azide. |
| Purification: | Affinity purified from tissue culture. |
| Concentration: | Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL. |
| Dilution Range: | ELISA (solid phase: 0, 1-100 µg/ml; tracer: 0, 001-100 µg/ml for 30 min at RT). Flow cytometry (0, 5-1, 0 ug/million cells in 0.1ml). Immunoblotting (1-2 µg/ml). Immunofluorescence (1-2 Mu g/ml). Inhibits tumor growth (order Ab without azide). Immuno |
| Storage Instruction: | Store for up to 1 year at 2-8°C upon receipt. |
| Gene Symbol: | TNF |
| Gene ID: | 7124 |
| Uniprot ID: | TNFA_HUMAN |
| Immunogen: | A BALB/c mouse was immunized with human rTNF-alpha. Fusion partner: NS-1. |
| Function | Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Up-regulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective. Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6. Promotes osteoclastogenesis and therefore mediates bone resorption. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells. |
| Protein Name | Tumor Necrosis Factor Cachectin Tnf-Alpha Tumor Necrosis Factor Ligand Superfamily Member 2 Tnf-A Cleaved Into - Tumor Necrosis Factor - Membrane Form N-Terminal Fragment Ntf - Intracellular Domain 1 Icd1 - Intracellular Domain 2 Icd2 - C-Domain 1 - C-Domain 2 - Tumor Necrosis Factor - Soluble Form |
| Database Links | Reactome: R-HSA-381340 Reactome: R-HSA-5357786 Reactome: R-HSA-5357905 Reactome: R-HSA-5357956 Reactome: R-HSA-5626978 Reactome: R-HSA-5668541 Reactome: R-HSA-6783783 Reactome: R-HSA-6785807 Reactome: R-HSA-75893 |
| Cellular Localisation | Cell Membrane Single-Pass Type Ii Membrane Protein Tumor Necrosis Factor Membrane Form: Membrane Soluble Form: Secreted C-Domain 1: Secreted C-Domain 2: Secreted |
| Alternative Antibody Names | Anti-Tumor Necrosis Factor antibody Anti-Cachectin antibody Anti-Tnf-Alpha antibody Anti-Tumor Necrosis Factor Ligand Superfamily Member 2 antibody Anti-Tnf-A Cleaved Into - Tumor Necrosis Factor - Membrane Form antibody Anti-N-Terminal Fragment antibody Anti-Ntf - Intracellular Domain 1 antibody Anti-Icd1 - Intracellular Domain 2 antibody Anti-Icd2 - C-Domain 1 - C-Domain 2 - Tumor Necrosis Factor - Soluble Form antibody Anti-TNF antibody Anti-TNFA antibody Anti-TNFSF2 antibody |
Information sourced from Uniprot.org