||WB, IHC-P, ELISA
||Rabbit polyclonal anti-TIF Alpha antibody is suitable for use in Western Blot, Immunohistochemistry and ELISA.
||FOR RESEARCH USE ONLY (RUO).
||Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
||The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
||Store at-20°C, and avoid repeat freeze-thaw cycles.
||WB 1:500-1:2000IHC 1:100-1:300ELISA 1:20000
||TIF1 alpha polyclonal antibody detects endogenous levels of TIF1 alpha protein.
||Synthesized peptide derived from human TIF1alpha
||960-1040 aa, C-terminal
| Function || Transcriptional coactivator that interacts with numerous nuclear receptors and coactivators and modulates the transcription of target genes. Interacts with chromatin depending on histone H3 modifications, having the highest affinity for histone H3 that is both unmodified at 'Lys-4' (H3K4me0) and acetylated at 'Lys-23' (H3K23ac). Has E3 protein-ubiquitin ligase activity. Promotes ubiquitination and proteasomal degradation of p53/TP53. Plays a role in the regulation of cell proliferation and apoptosis, at least in part via its effects on p53/TP53 levels. Up-regulates ligand-dependent transcription activation by AR, GCR/NR3C1, thyroid hormone receptor (TR) and ESR1. Modulates transcription activation by retinoic acid (RA) receptors, including RARA. Plays a role in regulating retinoic acid-dependent proliferation of hepatocytes. |
| Protein Name || Transcription Intermediary Factor 1-AlphaTif1-AlphaE3 Ubiquitin-Protein Ligase Trim24Ring Finger Protein 82Ring-Type E3 Ubiquitin Transferase Tif1-AlphaTripartite Motif-Containing Protein 24 |
| Database Links || Reactome: R-HSA-1839117Reactome: R-HSA-5655302Reactome: R-HSA-6802952 |
| Cellular Localisation || NucleusCytoplasmColocalizes With Sites Of Active TranscriptionDetected Both In Nucleus And Cytoplasm In Some Breast Cancer SamplesPredominantly Nuclear |
| Alternative Antibody Names || Anti-Transcription Intermediary Factor 1-Alpha antibodyAnti-Tif1-Alpha antibodyAnti-E3 Ubiquitin-Protein Ligase Trim24 antibodyAnti-Ring Finger Protein 82 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Tif1-Alpha antibodyAnti-Tripartite Motif-Containing Protein 24 antibodyAnti-TRIM24 antibodyAnti-RNF82 antibodyAnti-TIF1 antibodyAnti-TIF1A antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance