• Western blot analysis of the lysates from HepG2 cells using TRIM24 antibody.
  • Western blot analysis of lysates from COLO cells, using TRIM24 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of COLO205 cells using TIF1 Alpha Polyclonal Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000
  • Immunohistochemistry analysis of paraffin-embedded human brain tissue, using TRIM24 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using TIF1 Alpha Polyclonal Antibody diluted at 1:1000

Anti-TIF Alpha antibody (STJ96019)

SKU:
STJ96019

Current Stock:
Host: Rabbit
Applications: WB, IHC-P, ELISA
Reactivity: Human, Mouse
Short Description: Rabbit polyclonal anti-TIF Alpha antibody is suitable for use in Western Blot, Immunohistochemistry and ELISA.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/ml
Isotype: IgG
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
Specificity: TIF1 alpha polyclonal antibody detects endogenous levels of TIF1 alpha protein.
Uniprot ID: TIF1A_HUMAN
Gene ID: 8805
Gene Symbol: TRIM24
Immunogen: Synthesized peptide derived from human TIF1alpha
Immunogen Region: 960-1040 aa, C-terminal
Function Transcriptional coactivator that interacts with numerous nuclear receptors and coactivators and modulates the transcription of target genes. Interacts with chromatin depending on histone H3 modifications, having the highest affinity for histone H3 that is both unmodified at 'Lys-4' (H3K4me0) and acetylated at 'Lys-23' (H3K23ac). Has E3 protein-ubiquitin ligase activity. Promotes ubiquitination and proteasomal degradation of p53/TP53. Plays a role in the regulation of cell proliferation and apoptosis, at least in part via its effects on p53/TP53 levels. Up-regulates ligand-dependent transcription activation by AR, GCR/NR3C1, thyroid hormone receptor (TR) and ESR1. Modulates transcription activation by retinoic acid (RA) receptors, including RARA. Plays a role in regulating retinoic acid-dependent proliferation of hepatocytes.
Protein Name Transcription Intermediary Factor 1-Alpha
Tif1-Alpha
E3 Ubiquitin-Protein Ligase Trim24
Ring Finger Protein 82
Ring-Type E3 Ubiquitin Transferase Tif1-Alpha
Tripartite Motif-Containing Protein 24
Database Links Reactome: R-HSA-1839117
Reactome: R-HSA-5655302
Reactome: R-HSA-6802952
Cellular Localisation Nucleus
Cytoplasm
Colocalizes With Sites Of Active Transcription
Detected Both In Nucleus And Cytoplasm In Some Breast Cancer Samples
Predominantly Nuclear
Alternative Antibody Names Anti-Transcription Intermediary Factor 1-Alpha antibody
Anti-Tif1-Alpha antibody
Anti-E3 Ubiquitin-Protein Ligase Trim24 antibody
Anti-Ring Finger Protein 82 antibody
Anti-Ring-Type E3 Ubiquitin Transferase Tif1-Alpha antibody
Anti-Tripartite Motif-Containing Protein 24 antibody
Anti-TRIM24 antibody
Anti-RNF82 antibody
Anti-TIF1 antibody
Anti-TIF1A antibody

Information sourced from Uniprot.org

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