Biotinylated STJ72119 (0. 3µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-TGFBI, Biotinylated antibody (Internal) {Biotin} (STJ73495)

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STJ73495-100
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Host: Goat
Applications: Pep-ELISA/WB
Reactivity: Human/Mouse/Rat/Dog
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description : Goat polyclonal antibody anti-TGFBI, Biotinylated (Internal) is suitable for use in ELISA and Western Blot research applications.
Clonality : Polyclonal
Conjugation: Biotin
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: Peptide ELISA: antibody detection limit dilution 1:32000.
WB: Approx 70kDa band observed in Human Kidney lysates (calculated MW of 74.7kDa according to Human NP_000349.1). Recommended concentration: 0.2-0.5µg/ml.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: TGFBI
Gene ID: 7045
Uniprot ID: BGH3_HUMAN
Immunogen Region: Internal
Accession Number: NP_000349.1
Immunogen Sequence: QLYTDRTEKLRPE.
Post Translational Modifications Gamma-carboxylation is controversial. Gamma-carboxyglutamated.gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation.these residues may be required for binding to calcium. According to a more recent report, does not contain vitamin K-dependent gamma-carboxyglutamate residues. The EMI domain contains 2 expected intradomain disulfide bridges (Cys-49-Cys85 and Cys-84-Cys-97) and one unusual interdomain disulfide bridge to the second FAS1 domain (Cys-74-Cys-339). This arrangement violates the predicted disulfide bridge pattern of an EMI domain.
Function Plays a role in cell adhesion. May play a role in cell-collagen interactions.
Protein Name Transforming Growth Factor-Beta-Induced Protein Ig-H3
Beta Ig-H3
Kerato-Epithelin
Rgd-Containing Collagen-Associated Protein
Rgd-Cap
Database Links Reactome: R-HSA-977225
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
May Be Associated Both With Microfibrils And With The Cell Surface
Alternative Antibody Names Anti-Transforming Growth Factor-Beta-Induced Protein Ig-H3 antibody
Anti-Beta Ig-H3 antibody
Anti-Kerato-Epithelin antibody
Anti-Rgd-Containing Collagen-Associated Protein antibody
Anti-Rgd-Cap antibody
Anti-TGFBI antibody
Anti-BIGH3 antibody

Information sourced from Uniprot.org

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