• Western blot analysis of extracts of various cell lines, using TCTN3 antibody (STJ25800) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-TCTN3 antibody (200-475) (STJ25800)

SKU:
STJ25800

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TCTN3 (200-475) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TCTN3
Gene ID: 26123
Uniprot ID: TECT3_HUMAN
Immunogen Region: 200-475
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 200-475 of human TCTN3 (NP_056446.4).
Immunogen Sequence: QTQSPPSFYRAGDPILTYFP KWSVISLLRQPAGVGAGGLC AESNPAGFLESKSTTCTRFF KNLASSCTLDSALNAASYYN FTVLKVPRSMTDPQNMEFQV PVILTSQANAPLLAGNTCQN VVSQVTYEIETNGTFGIQKV SVSLGQTNLTVEPGASLQQH FILRFRAFQQSTAASLTSPR SGNPGYIVGKPLLALTDDIS YSMTLLQSQGNGSCSVKRHE VQFGVNAISGCKLRLKKAD
Function Part of the tectonic-like complex which is required for tissue-specific ciliogenesis and may regulate ciliary membrane composition. May be involved in apoptosis regulation. Necessary for signal transduction through the sonic hedgehog (Shh) signaling pathway.
Protein Name Tectonic-3
Database Links Reactome: R-HSA-5620912
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Tectonic-3 antibody
Anti-TCTN3 antibody
Anti-C10orf61 antibody
Anti-TECT3 antibody
Anti-PSEC0041 antibody
Anti-UNQ1881 antibody
Anti-PRO4324 antibody

Information sourced from Uniprot.org

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