• STJ72134 (2µg/ml) staining of Human Cerebellum (A) and Rat Lung (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ72134 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear and endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ72134 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-SYVN1 antibody (Internal) (STJ72134)

SKU:
STJ72134-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IF/FC
Reactivity: Human/Mouse/Rat/Dog
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-SYVN1 (Internal) is suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-1-3µg/ml
IF-Strong expression of the protein seen in the nuclei and endoplasmic reticulum of A431 cells. 10µg/ml
ELISA-antibody detection limit dilution 1:32000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: SYVN1
Gene ID: 84447
Uniprot ID: SYVN1_HUMAN
Immunogen Region: Internal
Accession Number: NP_115807.1; NP_757385.1
Specificity: This antibody is expected to recognize isoforms a and b (NP_115807.1; NP_757385.1).
Immunogen Sequence: HERQHLEARLQS
Post Translational Modifications Not N-glycosylated. Auto-ubiquitinated.
Function E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. Also promotes the degradation of normal but naturally short-lived proteins such as SGK. Protects cells from ER stress-induced apoptosis. Protects neurons from apoptosis induced by polyglutamine-expanded huntingtin (HTT) or unfolded GPR37 by promoting their degradation. Sequesters p53/TP53 in the cytoplasm and promotes its degradation, thereby negatively regulating its biological function in transcription, cell cycle regulation and apoptosis. Mediates the ubiquitination and subsequent degradation of cytoplasmic NFE2L1. During the early stage of B cell development, required for degradation of the pre-B cell receptor (pre-BCR) complex, hence supporting further differentiation into mature B cells.
Protein Name E3 Ubiquitin-Protein Ligase Synoviolin
Ring-Type E3 Ubiquitin Transferase Synoviolin
Synovial Apoptosis Inhibitor 1
Database Links Reactome: R-HSA-381038
Reactome: R-HSA-5358346
Reactome: R-HSA-5362768
Reactome: R-HSA-901032
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Synoviolin antibody
Anti-Ring-Type E3 Ubiquitin Transferase Synoviolin antibody
Anti-Synovial Apoptosis Inhibitor 1 antibody
Anti-SYVN1 antibody
Anti-HRD1 antibody
Anti-KIAA1810 antibody

Information sourced from Uniprot.org

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