• Western blot analysis of extracts of various cell lines, using STK16 antibody (STJ110465) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 15s.

Anti-STK16 antibody (1-305) (STJ110465)

SKU:
STJ110465

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-STK16 (1-305) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: STK16
Gene ID: 8576
Uniprot ID: STK16_HUMAN
Immunogen Region: 1-305
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-305 of human STK16 (NP_001008910.1).
Immunogen Sequence: MGHALCVCSRGTVIIDNKRY LFIQKLGEGGFSYVDLVEGL HDGHFYALKRILCHEQQDRE EAQREADMHRLFNHPNILRL VAYCLRERGAKHEAWLLLPF FKRGTLWNEIERLKDKGNFL TEDQILWLLLGICRGLEAIH AKGYAHRDLKPTNILLGDEG QPVLMDLGSMNQACIHVEGS RQALTLQDWAAQRCTISYRA PELFSVQSHCVIDERTDVWS LGCVLYAMMFGEGPYDMVF
Tissue Specificity Ubiquitously expressed at very low levels.
Post Translational Modifications Mainly autophosphorylated on serine/threonine residues. Also autophosphorylated on Tyr-198. It is uncertain whether palmitoylation is on Cys-6 and/or Cys-8.
Function Membrane-associated protein kinase that phosphorylates on serine and threonine residues. In vitro substrates include DRG1, ENO1 and EIF4EBP1. Also autophosphorylates. May be involved in secretory vesicle trafficking or intracellular signaling. May have a role in regulating stromal-epithelial interactions that occur during ductal morphogenesis in the mammary gland. May be involved in TGF-beta signaling. Able to autophosphorylate on Tyr residue.it is however unclear whether it has tyrosine-protein kinase toward other proteins.
Protein Name Serine/Threonine-Protein Kinase 16
Myristoylated And Palmitoylated Serine/Threonine-Protein Kinase
Mpsk
Protein Kinase Pkl12
Tgf-Beta-Stimulated Factor 1
Tsf-1
Tyrosine-Protein Kinase Stk16
Hpsk
Cellular Localisation Cytoplasm
Perinuclear Region
Membrane
Lipid-Anchor
Associates With Golgi And Golgi-Derived Vesicles
Alternative Antibody Names Anti-Serine/Threonine-Protein Kinase 16 antibody
Anti-Myristoylated And Palmitoylated Serine/Threonine-Protein Kinase antibody
Anti-Mpsk antibody
Anti-Protein Kinase Pkl12 antibody
Anti-Tgf-Beta-Stimulated Factor 1 antibody
Anti-Tsf-1 antibody
Anti-Tyrosine-Protein Kinase Stk16 antibody
Anti-Hpsk antibody
Anti-STK16 antibody
Anti-MPSK1 antibody
Anti-PKL12 antibody
Anti-TSF1 antibody

Information sourced from Uniprot.org

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