Anti-SQSTM1/p62 antibody (STJ110069)

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STJ110069

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Host: Rabbit
Applications: WB, IHC, IF, IP
Reactivity: Human, Mouse, Rat
Short Description: Rabbit polyclonal anti-SQSTM1/p62 antibody is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:500-1:2000
IHC: 1:50-1:200
IF: 1:50-1:200
IP: 1:50-1:100
Uniprot ID: SQSTM_HUMAN
Gene ID: 8878
Gene Symbol: SQSTM1
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-440 of human SQSTM1/p62 (NP_001135770.1).
Tissue Specificity Ubiquitously expressed.
Post Translational Modifications Phosphorylated. May be phosphorylated by PRKCZ. Phosphorylated in vitro by TTN. Phosphorylation at Ser-403 by ULK1 is stimulated by SESN2. Phosphorylated at Ser-403 by TBK1, leading to promote relocalization of 'Lys-63'-linked ubiquitinated STING1 to autophagosomes. Phosphorylation at Ser-349 by MTOR promotes interaction with KEAP1 and inactivation of the BCR(KEAP1) complex, promoting NFE2L2/NRF2 nuclear accumulation and expression of phase II detoxifying enzymes. Ubiquitinated by RNF26: ubiquitinated SQSTM1 attracts specific vesicle-associated adapters, forming a molecular bridge that restrains cognate vesicles in the perinuclear region and organizes the endosomal pathway for efficient cargo transport. Deubiquitination by USP15 releases target vesicles for fast transport into the cell periphery. Ubiquitinated by the BCR(KEAP1) complex at Lys-420, increasing SQSTM1 sequestering activity and promoting its degradation. Ubiquitinated via 'Lys-29' and 'Lys-33'-linked polyubiquitination leading to xenophagic targeting of bacteria and inhibition of their replication.
Function Autophagy receptor required for selective macroautophagy (aggrephagy). Functions as a bridge between polyubiquitinated cargo and autophagosomes. Interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family. Along with WDFY3, involved in the formation and autophagic degradation of cytoplasmic ubiquitin-containing inclusions (p62 bodies, ALIS/aggresome-like induced structures). Along with WDFY3, required to recruit ubiquitinated proteins to PML bodies in the nucleus. May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD. May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels. Involved in endosome organization by retaining vesicles in the perinuclear cloud: following ubiquitination by RNF26, attracts specific vesicle-associated adapters, forming a molecular bridge that restrains cognate vesicles in the perinuclear region and organizes the endosomal pathway for efficient cargo transport. Promotes relocalization of 'Lys-63'-linked ubiquitinated STING1 to autophagosomes. Acts as an activator of the NFE2L2/NRF2 pathway via interaction with KEAP1: interaction inactivates the BCR(KEAP1) complex, promoting nuclear accumulation of NFE2L2/NRF2 and subsequent expression of cytoprotective genes.
Protein Name Sequestosome-1
Ebi3-Associated Protein Of 60 Kda
Ebiap
P60
Phosphotyrosine-Independent Ligand For The Lck Sh2 Domain Of 62 Kda
Ubiquitin-Binding Protein P62
Database Links Reactome: R-HSA-205043
Reactome: R-HSA-209543
Reactome: R-HSA-209560
Reactome: R-HSA-5205685
Reactome: R-HSA-9020702
Reactome: R-HSA-9664873
Cellular Localisation Cytoplasm
Cytosol
Late Endosome
Lysosome
Cytoplasmic Vesicle
Autophagosome
Nucleus
Endoplasmic Reticulum
Pml Body
Myofibril
Sarcomere
In Cardiac Muscle
Localizes To The Sarcomeric Band
Commonly Found In Inclusion Bodies Containing Polyubiquitinated Protein Aggregates
In Neurodegenerative Diseases
Detected In Lewy Bodies In Parkinson Disease
Neurofibrillary Tangles In Alzheimer Disease
And Htt Aggregates In Huntington Disease
In Protein Aggregate Diseases Of The Liver
Found In Large Amounts In Mallory Bodies Of Alcoholic And Nonalcoholic Steatohepatitis
Hyaline Bodies In Hepatocellular Carcinoma
And In Serpina1 Aggregates
Enriched In Rosenthal Fibers Of Pilocytic Astrocytoma
In The Cytoplasm
Observed In Both Membrane-Free Ubiquitin-Containing Protein Aggregates (Sequestosomes) And Membrane-Surrounded Autophagosomes
Colocalizes With Trim13 In The Perinuclear Endoplasmic Reticulum
Co-Localizes With Trim5 In Cytoplasmic Bodies
When Nuclear Export Is Blocked By Treatment With Leptomycin B
Accumulates In Pml Bodies
Alternative Antibody Names Anti-Sequestosome-1 antibody
Anti-Ebi3-Associated Protein Of 60 Kda antibody
Anti-Ebiap antibody
Anti-P60 antibody
Anti-Phosphotyrosine-Independent Ligand For The Lck Sh2 Domain Of 62 Kda antibody
Anti-Ubiquitin-Binding Protein P62 antibody
Anti-SQSTM1 antibody
Anti-ORCA antibody
Anti-OSIL antibody

Information sourced from Uniprot.org

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