• Immunohistochemical analysis of paraffin-embedded mouse-brain, antibody was diluted at 1:100
  • Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100
  • Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100
  • Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100
  • Western blot analysis of 293T SH-SY5Y lysis using SPTLC1 antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000
  • Western blot analysis of HepG2 cells using SPTLC1 Polyclonal Antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000

Anti-SPTLC1 antibody (411-460 aa) (STJ96597)

SKU:
STJ96597

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Serine palmitoyltransferase 1 (411-460 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-300
ELISA 1:20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SPTLC1
Gene ID: 10558
Uniprot ID: SPTC1_HUMAN
Immunogen Region: 411-460 aa
Specificity: SPTLC1 Polyclonal Antibody detects endogenous levels of SPTLC1 protein.
Immunogen: Synthesized peptide derived from SPTLC1 at the amino acid range 411-460
Post Translational Modifications Phosphorylation at Tyr-164 inhibits activity and promotes cell survival.
Function Component of the serine palmitoyltransferase multisubunit enzyme (SPT) that catalyzes the initial and rate-limiting step in sphingolipid biosynthesis by condensing L-serine and activated acyl-CoA (most commonly palmitoyl-CoA) to form long-chain bases. The SPT complex is also composed of SPTLC2 or SPTLC3 and SPTSSA or SPTSSB. Within this complex, the heterodimer with SPTLC2 or SPTLC3 forms the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates, with a slight preference for C14-CoA. The SPTLC1-SPTLC2-SPTSSB complex shows a strong preference for C18-CoA substrate, while the SPTLC1-SPTLC3-SPTSSB isozyme displays an ability to use a broader range of acyl-CoAs, without apparent preference. Required for adipocyte cell viability and metabolic homeostasis.
Protein Name Serine Palmitoyltransferase 1
Long Chain Base Biosynthesis Protein 1
Lcb 1
Serine-Palmitoyl-Coa Transferase 1
Spt 1
Spt1
Database Links Reactome: R-HSA-1660661
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Membrane Protein
Alternative Antibody Names Anti-Serine Palmitoyltransferase 1 antibody
Anti-Long Chain Base Biosynthesis Protein 1 antibody
Anti-Lcb 1 antibody
Anti-Serine-Palmitoyl-Coa Transferase 1 antibody
Anti-Spt 1 antibody
Anti-Spt1 antibody
Anti-SPTLC1 antibody
Anti-LCB1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance