• Western blot analysis of extracts of various cell lines, using SOX2 antibody (STJ113816) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of C6 cells using SOX2 Polyclonal Antibody (STJ113816) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using SOX2 Polyclonal Antibody (STJ113816) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Confocal immunofluorescence analysis of U-2 OS cells using SOX2 Polyclonal Antibody (STJ113816) at dilution of 1:200. Blue: DAPI for nuclear staining.

Anti-SOX2 antibody (1-140) (STJ113816)

SKU:
STJ113816

£160.50 - £359.50
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Host: Rabbit
Applications: WB, IF
Reactivity: Human, Mouse, Rat
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Rabbit polyclonal antibody anti-SOX2 (1-140) is suitable for use in Western Blot and Immunofluorescence.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS containing 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF 1:50-1:200
Storage Instruction: Store in a freezer at-20°C and avoid freeze-thaw cycles.
Gene Symbol: SOX2
Gene ID: 6657
Uniprot ID: SOX2_HUMAN
Immunogen Region: 1-140
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human SOX2 (NP_003097.1).
Post Translational Modifications Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.
Function Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Binds to the proximal enhancer region of NANOG. Critical for early embryogenesis and for embryonic stem cell pluripotency. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation. May function as a switch in neuronal development.
Protein Name Transcription Factor Sox-2
Database Links Reactome: R-HSA-2892245
Reactome: R-HSA-2892247
Reactome: R-HSA-3769402
Reactome: R-HSA-452723
Reactome: R-HSA-6785807
Reactome: R-HSA-8986944
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Transcription Factor Sox-2 antibody
Anti-SOX2 antibody

Information sourced from Uniprot.org

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