| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-14-3-3 protein sigma (41-90 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | IHC-P 100-300 WB 1:500-1:2000 ELISA 1:20000 IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | SFN |
| Gene ID: | 2810 |
| Uniprot ID: | 1433S_HUMAN |
| Immunogen Region: | 41-90 aa |
| Specificity: | 14-3-3 Sigma Polyclonal Antibody detects endogenous levels of 14-3-3 Sigma protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human SFN at the amino acid range 41-90 |
| Post Translational Modifications | Ubiquitinated. Ubiquitination by RFFL induces proteasomal degradation and indirectly regulates p53/TP53 activation. |
| Function | Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Promotes cytosolic retention of GBP1 GTPase by binding to phosphorylated GBP1, thereby inhibiting the innate immune response. Also acts as a TP53/p53-regulated inhibitor of G2/M progression. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. Acts to maintain desmosome cell junction adhesion in epithelial cells via interacting with and sequestering PKP3 to the cytoplasm, thereby restricting its translocation to existing desmosome structures and therefore maintaining desmosome protein homeostasis. Also acts to facilitate PKP3 exchange at desmosome plaques, thereby maintaining keratinocyte intercellular adhesion. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53. |
| Protein Name | 14-3-3 Protein Sigma Epithelial Cell Marker Protein 1 Stratifin |
| Database Links | Reactome: R-HSA-111447 Reactome: R-HSA-1445148 Reactome: R-HSA-5625740 Reactome: R-HSA-5628897 Reactome: R-HSA-6804114 Reactome: R-HSA-75035 Reactome: R-HSA-9614399 Reactome: R-HSA-9735871 Reactome: R-HSA-9755779 |
| Cellular Localisation | Cytoplasm Nucleus Secreted May Be Secreted By A Non-Classical Secretory Pathway |
| Alternative Antibody Names | Anti-14-3-3 Protein Sigma antibody Anti-Epithelial Cell Marker Protein 1 antibody Anti-Stratifin antibody Anti-SFN antibody Anti-HME1 antibody |
Information sourced from Uniprot.org