| Host: | Rabbit |
| Applications: | WB/IHC-P/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:100-1:500 IHC-P:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | RPL5 |
| Gene ID: | 6125 |
| Uniprot ID: | RL5_HUMAN |
| Immunogen Region: | 1-95 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-95 of human RPL5 (NP_000960.2). |
| Immunogen Sequence: | MGFVKVVKNKAYFKRYQVKF RRRREGKTDYYARKRLVIQD KNKYNTPKYRMIVRVTNRDI ICQIAYARIEGDMIVCAAYA HELPKYGVKVGLTNY |
| Function | Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. As part of the 5S RNP/5S ribonucleoprotein particle it is an essential component of the LSU, required for its formation and the maturation of rRNAs. It also couples ribosome biogenesis to p53/TP53 activation. As part of the 5S RNP it accumulates in the nucleoplasm and inhibits MDM2, when ribosome biogenesis is perturbed, mediating the stabilization and the activation of TP53. |
| Protein Name | Large Ribosomal Subunit Protein Ul18 60s Ribosomal Protein L5 |
| Database Links | Reactome: R-HSA-156827 Reactome: R-HSA-156902 Reactome: R-HSA-1799339 Reactome: R-HSA-192823 Reactome: R-HSA-2408557 Reactome: R-HSA-6791226 Reactome: R-HSA-72689 Reactome: R-HSA-72706 Reactome: R-HSA-72764 Reactome: R-HSA-9010553 Reactome: R-HSA-9633012 Reactome: R-HSA-975956 Reactome: R-HSA-975957 |
| Cellular Localisation | Cytoplasm Nucleus Nucleolus Although Rp5 Is Functional Within The Cytoplasm The Assembly Of Ribosomal Subunits Occurs In The Nucleus Rpl5 Nuclear Import Is Mediated By Ipo5/Ranbp5 Ipo7/Ranbp7 Kpnb1/Importin-Beta Or Tpno1/Trn |
| Alternative Antibody Names | Anti-Large Ribosomal Subunit Protein Ul18 antibody Anti-60s Ribosomal Protein L5 antibody Anti-RPL5 antibody Anti-MSTP030 antibody |
Information sourced from Uniprot.org