• Immunohistochemistry analysis of paraffin-embedded mouse heart using RPH3A antibody (STJ115545) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using RPH3A antibody (STJ115545) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using RPH3A antibody (STJ115545) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using RPH3A antibody (STJ115545) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-RPH3A antibody (1-280) (STJ115545)

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STJ115545

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RPH3A (1-280) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RPH3A
Gene ID: 22895
Uniprot ID: RP3A_HUMAN
Immunogen Region: 1-280
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human RPH3A (NP_055769.2).
Immunogen Sequence: MTDTVFSNSSNRWMYPSDRP LQSKLQAGWSVHPGGQPDRQ RKQEELTDEEKEIINRVIAR AEKMEEMEQERIGRLVDRLE NMRKNVAGDGVNRCILCGEQ LGMLGSACVVCEDCKKNVCT KCGVETNNRLHSVWLCKICI EQREVWKRSGAWFFKGFPKQ VLPQPMPIKKTKPQQPVSEP AAPEQPAPEPKHPARAPARG DSEDRRGPGQKTGPDPASAP GRGNYGPPVRRASEARMSS
Post Translational Modifications Ubiquitinated. Deubiquitinated by CAND1 to prevent its degradation.
Function Plays an essential role in docking and fusion steps of regulated exocytosis. At the presynaptic level, RPH3A is recruited by RAB3A to the synaptic vesicle membrane in a GTP-dependent manner where it modulates synaptic vesicle trafficking and calcium-triggered neurotransmitter release. In the post-synaptic compartment, forms a ternary complex with GRIN2A and DLG4 and regulates NMDA receptor stability. Also plays a role in the exocytosis of arginine vasopressin hormone.
Protein Name Rabphilin-3a
Exophilin-1
Cellular Localisation Cytoplasmic Vesicle
Secretory Vesicle
Synaptic Vesicle Membrane
Cell Projection
Dendritic Spine
Postsynaptic Cell Membrane
Membrane
Peripheral Membrane Protein
Alternative Antibody Names Anti-Rabphilin-3a antibody
Anti-Exophilin-1 antibody
Anti-RPH3A antibody
Anti-KIAA0985 antibody

Information sourced from Uniprot.org

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