Anti-RPE65 antibody (320-370 aa) (STJ13100232)

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STJ13100232-100
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Host: Rabbit
Applications: IHC/WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description : Rabbit polyclonal antibody anti-RPE65 (320-370 aa) is suitable for use in Immunohistochemistry and Western Blot research applications.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Lyophilised
Purification: IgG purified
Dilution Range: IHC, WB (confirmed by recombinant protein). A dilution of 1:300 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
Storage Instruction: Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Gene Symbol: RPE65
Gene ID: 6121
Uniprot ID: RPE65_HUMAN
Immunogen Region: 320-370 aa
Specificity: Specific for RPE65.
Immunogen: A synthetic peptide from aa region 320-370 of human RPE65 conjugated to an immunogenic carrier protein was used as the antigen.
Post Translational Modifications Palmitoylation by LRAT regulates ligand binding specificity.the palmitoylated form (membrane form) specifically binds all-trans-retinyl-palmitate, while the soluble unpalmitoylated form binds all-trans-retinol (vitamin A).
Function Critical isomerohydrolase in the retinoid cycle involved in regeneration of 11-cis-retinal, the chromophore of rod and cone opsins. Catalyzes the cleavage and isomerization of all-trans-retinyl fatty acid esters to 11-cis-retinol which is further oxidized by 11-cis retinol dehydrogenase to 11-cis-retinal for use as visual chromophore. Essential for the production of 11-cis retinal for both rod and cone photoreceptors. Also capable of catalyzing the isomerization of lutein to meso-zeaxanthin an eye-specific carotenoid. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT.
Protein Name Retinoid Isomerohydrolase
All-Trans-Retinyl-Palmitate Hydrolase
Lutein Isomerase
Meso-Zeaxanthin Isomerase
Retinal Pigment Epithelium-Specific 65 Kda Protein
Retinol Isomerase
Database Links Reactome: R-HSA-2453902
Cellular Localisation Cytoplasm
Cell Membrane
Lipid-Anchor
Microsome Membrane
Attached To The Membrane By A Lipid Anchor When Palmitoylated (Membrane Form)
Soluble When Unpalmitoylated
Undergoes Light-Dependent Intracellular Transport To Become More Concentrated In The Central Region Of The Retina Pigment Epithelium Cells
Alternative Antibody Names Anti-Retinoid Isomerohydrolase antibody
Anti-All-Trans-Retinyl-Palmitate Hydrolase antibody
Anti-Lutein Isomerase antibody
Anti-Meso-Zeaxanthin Isomerase antibody
Anti-Retinal Pigment Epithelium-Specific 65 Kda Protein antibody
Anti-Retinol Isomerase antibody
Anti-RPE65 antibody

Information sourced from Uniprot.org

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