| Host: | Rabbit |
| Applications: | IHC/WB |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-RPE65 (320-370 aa) is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Lyophilised |
| Purification: | IgG purified |
| Dilution Range: | IHC, WB (confirmed by recombinant protein). A dilution of 1:300 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Storage Instruction: | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: | RPE65 |
| Gene ID: | 6121 |
| Uniprot ID: | RPE65_HUMAN |
| Immunogen Region: | 320-370 aa |
| Specificity: | Specific for RPE65. |
| Immunogen: | A synthetic peptide from aa region 320-370 of human RPE65 conjugated to an immunogenic carrier protein was used as the antigen. |
| Post Translational Modifications | Palmitoylation by LRAT regulates ligand binding specificity.the palmitoylated form (membrane form) specifically binds all-trans-retinyl-palmitate, while the soluble unpalmitoylated form binds all-trans-retinol (vitamin A). |
| Function | Critical isomerohydrolase in the retinoid cycle involved in regeneration of 11-cis-retinal, the chromophore of rod and cone opsins. Catalyzes the cleavage and isomerization of all-trans-retinyl fatty acid esters to 11-cis-retinol which is further oxidized by 11-cis retinol dehydrogenase to 11-cis-retinal for use as visual chromophore. Essential for the production of 11-cis retinal for both rod and cone photoreceptors. Also capable of catalyzing the isomerization of lutein to meso-zeaxanthin an eye-specific carotenoid. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT. |
| Protein Name | Retinoid Isomerohydrolase All-Trans-Retinyl-Palmitate Hydrolase Lutein Isomerase Meso-Zeaxanthin Isomerase Retinal Pigment Epithelium-Specific 65 Kda Protein Retinol Isomerase |
| Database Links | Reactome: R-HSA-2453902 |
| Cellular Localisation | Cytoplasm Cell Membrane Lipid-Anchor Microsome Membrane Attached To The Membrane By A Lipid Anchor When Palmitoylated (Membrane Form) Soluble When Unpalmitoylated Undergoes Light-Dependent Intracellular Transport To Become More Concentrated In The Central Region Of The Retina Pigment Epithelium Cells |
| Alternative Antibody Names | Anti-Retinoid Isomerohydrolase antibody Anti-All-Trans-Retinyl-Palmitate Hydrolase antibody Anti-Lutein Isomerase antibody Anti-Meso-Zeaxanthin Isomerase antibody Anti-Retinal Pigment Epithelium-Specific 65 Kda Protein antibody Anti-Retinol Isomerase antibody Anti-RPE65 antibody |
Information sourced from Uniprot.org