| Host: | Rabbit |
| Applications: | WB |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-E3 ubiquitin-protein ligase RNF31 (833-883) is suitable for use in Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:1000-2000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | RNF31 |
| Gene ID: | 55072 |
| Uniprot ID: | RNF31_HUMAN |
| Immunogen Region: | 833-883 |
| Specificity: | This antibody detects endogenous levels of human RNF31 |
| Immunogen: | Synthesized peptide derived from human RNF31 AA range: 833-883 |
| Post Translational Modifications | Autoubiquitinated. Interaction with OTULIN is required to suppress formation of 'Met-1'-linked polyubiquitin chains and prevent subsequent inactivation of the LUBAC complex. Cleaved by caspase during apoptosis. (Microbial infection) Ubiquitinated by S.flexneri E3 ubiquitin-protein ligases IpaH1.4 and IpaH2.5, leading to its degradation by the proteasome, thereby preventing formation of the bacterial ubiquitin coat and activation of innate immunity. |
| Function | E3 ubiquitin-protein ligase component of the LUBAC complex which conjugates linear ('Met-1'-linked) polyubiquitin chains to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. The LUBAC complex is also involved in innate immunity by conjugating linear polyubiquitin chains at the surface of bacteria invading the cytosol to form the ubiquitin coat surrounding bacteria. LUBAC is not able to initiate formation of the bacterial ubiquitin coat, and can only promote formation of linear polyubiquitins on pre-existing ubiquitin. Recruited to the surface of bacteria by RNF213, which initiates the bacterial ubiquitin coat. The bacterial ubiquitin coat acts as an 'eat-me' signal for xenophagy and promotes NF-kappa-B activation. Together with OTULIN, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis. RNF31 is required for linear ubiquitination of BCL10, thereby promoting TCR-induced NF-kappa-B activation. Binds polyubiquitin of different linkage types. |
| Protein Name | E3 Ubiquitin-Protein Ligase Rnf31 Hoil-1-Interacting Protein Hoip Ring Finger Protein 31 Ring-Type E3 Ubiquitin Transferase Rnf31 Zinc In-Between-Ring-Finger Ubiquitin-Associated Domain Protein |
| Database Links | Reactome: R-HSA-5357786 Reactome: R-HSA-5357905 Reactome: R-HSA-5357956 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Rnf31 antibody Anti-Hoil-1-Interacting Protein antibody Anti-Hoip antibody Anti-Ring Finger Protein 31 antibody Anti-Ring-Type E3 Ubiquitin Transferase Rnf31 antibody Anti-Zinc In-Between-Ring-Finger Ubiquitin-Associated Domain Protein antibody Anti-RNF31 antibody Anti-ZIBRA antibody |
Information sourced from Uniprot.org