| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | RNF14 |
| Gene ID: | 9604 |
| Uniprot ID: | RNF14_HUMAN |
| Immunogen Region: | 125-474 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 125-474 of human RNF14 (NP_004281.1). |
| Immunogen Sequence: | AWMQFLKEETLAYLNIVSPF ELKIGSQKKVQRRTAQASPN TELDFGGAAGSDVDQEEIVD ERAVQDVESLSNLIQEILDF DQAQQIKCFNSKLFLCSICF CEKLGSECMYFLECRHVYCK ACLKDYFEIQIRDGQVQCLN CPEPKCPSVATPGQVKELVE AELFARYDRLLLQSSLDLMA DVVYCPRPCCQLPVMQEPGC TMGICSSCNFAFCTLCRLTY HGVSPCKVTAEKLMDLRNE |
| Tissue Specificity | Widely expressed. |
| Post Translational Modifications | RING-type zinc finger-dependent and UBE2E2-dependent autoubiquitination. |
| Function | E3 ubiquitin-protein ligase that plays a key role in the RNF14-RNF25 translation quality control pathway, a pathway that takes place when a ribosome has stalled during translation, and which promotes ubiquitination and degradation of translation factors on stalled ribosomes. Recruited to stalled ribosomes by the ribosome collision sensor GCN1 and mediates 'Lys-6'-linked ubiquitination of target proteins, leading to their degradation. Mediates ubiquitination of EEF1A1/eEF1A and ETF1/eRF1 translation factors on stalled ribosomes, leading to their degradation. Also catalyzes ubiquitination of ribosomal proteins RPL0, RPL1, RPL12, RPS13 and RPS17. Specifically required to resolve RNA-protein cross-links caused by reactive aldehydes, which trigger translation stress by stalling ribosomes: acts by catalying 'Lys-6'-linked ubiquitination of RNA-protein cross-links, leading to their removal by the ATP-dependent unfoldase VCP and subsequent degradation by the proteasome. Independently of its function in the response to stalled ribosomes, acts as a regulator of transcription in Wnt signaling via its interaction with TCF transcription factors (TCF7/TCF1, TCF7L1/TCF3 and TCF7L2/TCF4). May also play a role as a coactivator for androgen- and, to a lesser extent, progesterone-dependent transcription. |
| Protein Name | E3 Ubiquitin-Protein Ligase Rnf14 Androgen Receptor-Associated Protein 54 Hfb30 Ring Finger Protein 14 |
| Database Links | Reactome: R-HSA-983168 |
| Cellular Localisation | Cytoplasm Nucleus |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Rnf14 antibody Anti-Androgen Receptor-Associated Protein 54 antibody Anti-Hfb30 antibody Anti-Ring Finger Protein 14 antibody Anti-RNF14 antibody Anti-ARA54 antibody Anti-HRIHFB2038 antibody |
Information sourced from Uniprot.org