| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human/Rat/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-E3 ubiquitin-protein ligase RNF135 (81-130 aa) is suitable for use in Western Blot and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-2000ELISA 1:5000-20000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RNF135 |
| Gene ID: |
84282 |
| Uniprot ID: |
RN135_HUMAN |
| Immunogen Region: |
81-130 aa |
| Specificity: |
RN135 Polyclonal Antibody detects endogenous levels of protein. |
| Immunogen: |
Synthesized peptide derived from the human protein at the amino acid range 81-130 |
| Post Translational Modifications | (Microbial infection) Cleaved and inactivated by hepatitis C virus NS3/NS4A. |
| Function | E2-dependent E3 ubiquitin-protein ligase that functions as a RIGI coreceptor in the sensing of viral RNAs in cell cytoplasm and the activation of the antiviral innate immune response. Together with the UBE2D3, UBE2N and UB2V1 E2 ligases, catalyzes the 'Lys-63'-linked polyubiquitination of RIGI oligomerized on viral RNAs, an essential step in the activation of the RIG-I signaling pathway. Through a ubiquitin-independent parallel mechanism, which consists in bridging RIGI filaments forming on longer viral RNAs, further activates the RIG-I signaling pathway. This second mechanism that synergizes with the ubiquitin-dependent one would thereby allow an RNA length-dependent regulation of the RIG-I signaling pathway (Probable). Associated with the E2 ligase UBE2N, also constitutively synthesizes unanchored 'Lys-63'-linked polyubiquitin chains that may also activate the RIG-I signaling pathway. |
| Protein Name | E3 Ubiquitin-Protein Ligase Rnf135Rig-I E3 Ubiquitin LigaseReulRing Finger Protein 135Ring Finger Protein Leading To Rig-I ActivationRipletRing-Type E3 Ubiquitin Transferase Rnf135 |
| Database Links | Reactome: R-HSA-168928Reactome: R-HSA-5689896Reactome: R-HSA-918233Reactome: R-HSA-933541Reactome: R-HSA-933542Reactome: R-HSA-933543Reactome: R-HSA-936440Reactome: R-HSA-9705671 |
| Cellular Localisation | CytoplasmStress Granule |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Rnf135 antibodyAnti-Rig-I E3 Ubiquitin Ligase antibodyAnti-Reul antibodyAnti-Ring Finger Protein 135 antibodyAnti-Ring Finger Protein Leading To Rig-I Activation antibodyAnti-Riplet antibodyAnti-Ring-Type E3 Ubiquitin Transferase Rnf135 antibodyAnti-RNF135 antibodyAnti-L13 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
