| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-RIF1 (2173-2472) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RIF1 |
| Gene ID: |
55183 |
| Uniprot ID: |
RIF1_HUMAN |
| Immunogen Region: |
2173-2472 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 2173-2472 of human RIF1 (NP_060621.3). |
| Immunogen Sequence: |
PLASPSTSILKRGLKRSQED EISSPVNKVRRVSFADPIYQ AGLADDIDRRCSIVRSHSSN SSPIGKSVKTSPTTQSKHNT TSAKGFLSPGSRSPKFKSSK KCLISEMAKESIPCPTESVY PPLVNCVAPVDIILPQITSN MWARGLGQLIRAKNIKTIGD LSTLTASEIKTLPIRSPKVS NVKKALRIYHEQQVKTRGLE EIPVFDISEKTVNGIENKSL SPDEERLVSDIIDPVALEI |
| Tissue Specificity | Highly expressed in testis. |
| Function | Key regulator of TP53BP1 that plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage: acts by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs. In response to DNA damage, interacts with ATM-phosphorylated TP53BP1. Interaction with TP53BP1 leads to dissociate the interaction between NUDT16L1/TIRR and TP53BP1, thereby unmasking the tandem Tudor-like domain of TP53BP1 and allowing recruitment to DNA DSBs. Once recruited to DSBs, RIF1 and TP53BP1 act by promoting NHEJ-mediated repair of DSBs. In the same time, RIF1 and TP53BP1 specifically counteract the function of BRCA1 by blocking DSBs resection via homologous recombination (HR) during G1 phase. Also required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs. Promotes NHEJ of dysfunctional telomeres. |
| Protein Name | Telomere-Associated Protein Rif1Rap1-Interacting Factor 1 Homolog |
| Database Links | Reactome: R-HSA-5693571 |
| Cellular Localisation | NucleusChromosomeTelomereCytoplasmCytoskeletonSpindleFollowing Interaction With Tp53bp1Recruited To Sites Of Dna DamageSuch As DsbsExhibits Atm- And Tp53bp1-Dependent Localization To Uncapped Or Aberrant Telomeres And To Dna Double Strand Breaks (Dsbs)Does Not Associate With Normal Telomere StructuresLocalizes To Microtubules Of The Midzone Of The Mitotic Spindle During AnaphaseAnd To Condensed Chromosomes In Telophase |
| Alternative Antibody Names | Anti-Telomere-Associated Protein Rif1 antibodyAnti-Rap1-Interacting Factor 1 Homolog antibodyAnti-RIF1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
