• Western blot analysis of extracts of various cell lines, using RGMA antibody (STJ25343) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.

Anti-RGMA antibody (STJ25343)

SKU:
STJ25343
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Host: Rabbit
Applications: WB
Reactivity: Human
Short Description: Rabbit polyclonal primary antibody against RGMA.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:500-1:2000
Uniprot ID: RGMA_HUMAN
Gene ID: 56963
Gene Symbol: RGMA
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 101-450 of human RGMA (NP_064596.2).
Background This gene encodes a member of the repulsive guidance molecule family. The encoded protein is a glycosylphosphatidylinositol-anchored glycoprotein that functions as an axon guidance protein in the developing and adult central nervous system. This protein may also function as a tumor suppressor in some cancers. Alternate splicing results in multiple transcript variants.
Post Translational Modifications Autocatalytically cleaved at low pH.the two chains remain linked via two disulfide bonds.
Function Member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. Regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. Binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition. Furthermore, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS-PTK2/FAK1-AKT1 pathway. It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8.
Database Links Reactome: R-HSA-373752
Cellular Localisation Cell Membrane
Lipid-Anchor
Gpi-Anchor
Protein Name Repulsive Guidance Molecule A
Rgm Domain Family Member A
Alternative Names Anti-Repulsive Guidance Molecule A antibody
Anti-Rgm Domain Family Member A antibody
Anti-RGMA antibody
Anti-RGM antibody

Information sourced from uniprot.org

6 months standard