| Post Translational Modifications | Phosphorylated at Ser-46 and Ser-63 upon DNA damage by ATM or ATR. ATM phosphorylation occurs at early times upon DNA damage, while ATR is the major kinase at later times. Phosphorylation by ATM and ATR is required to stabilize p53/TP53. Part of the phosphorylation depends upon RPA2 presence. |
| Function | E3 ubiquitin-protein ligase required for the repair of DNA interstrand cross-links (ICL) in response to DNA damage. Plays a key role in RPA-mediated DNA damage signaling and repair. Acts by mediating ubiquitination of the RPA complex (RPA1, RPA2 and RPA3 subunits) and RAD51 at stalled replication forks, leading to remove them from DNA damage sites and promote homologous recombination. Also mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. May act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. In response to ionizing radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53. Required to translesion DNA synthesis across DNA-protein cross-link adducts by catalyzing ubiquitination of proteins on single-stranded DNA (ssDNA). |
| Protein Name | E3 Ubiquitin-Protein Ligase Rfwd3Ring Finger And Wd Repeat Domain-Containing Protein 3Ring Finger Protein 201 |
| Cellular Localisation | NucleusPml BodyCytoplasmIn Undamaged CellsFound Both In The Cytoplasm And In The NucleusPartially Associated With Pml Nuclear BodiesIn Response To Replication BlockSuch As That Caused By Hydroxyurea TreatmentOr To Dna Damage Caused By Ionizing Radiations Or DoxorubicinRecruited To The NucleusTo Stalled Replication Forks Or To Sites Of Dna RepairThis Recruitment Depends Upon Rpa2 |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Rfwd3 antibodyAnti-Ring Finger And Wd Repeat Domain-Containing Protein 3 antibodyAnti-Ring Finger Protein 201 antibodyAnti-RFWD3 antibodyAnti-RNF201 antibody |
Information sourced from Uniprot.org
