| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-RNA-binding protein 4 is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RBM4 |
| Gene ID: |
5936 |
| Uniprot ID: |
RBM4_HUMAN |
| Specificity: |
This antibody detects endogenous levels of RBM4 at Human, Mouse |
| Immunogen: |
Synthesized peptide derived from human RBM4 |
| Post Translational Modifications | Phosphorylation on Ser-309 is induced upon cell muscle differentiation. Phosphorylated. Phosphorylated in vitro on Ser-309 by SRPK1. Phosphorylation on Ser-309 is induced upon cell stress signaling, which alters its subcellular localization and may modulate its activity on IRES-mediated mRNA translation. |
| Function | RNA-binding factor involved in multiple aspects of cellular processes like alternative splicing of pre-mRNA and translation regulation. Modulates alternative 5'-splice site and exon selection. Acts as a muscle cell differentiation-promoting factor. Activates exon skipping of the PTB pre-mRNA during muscle cell differentiation. Antagonizes the activity of the splicing factor PTBP1 to modulate muscle cell-specific exon selection of alpha tropomyosin. Binds to intronic pyrimidine-rich sequence of the TPM1 and MAPT pre-mRNAs. Required for the translational activation of PER1 mRNA in response to circadian clock. Binds directly to the 3'-UTR of the PER1 mRNA. Exerts a suppressive activity on Cap-dependent translation via binding to CU-rich responsive elements within the 3'UTR of mRNAs, a process increased under stress conditions or during myocytes differentiation. Recruits EIF4A1 to stimulate IRES-dependent translation initiation in respons to cellular stress. Associates to internal ribosome entry segment (IRES) in target mRNA species under stress conditions. Plays a role for miRNA-guided RNA cleavage and translation suppression by promoting association of AGO2-containing miRNPs with their cognate target mRNAs. Associates with miRNAs during muscle cell differentiation. Binds preferentially to 5'-CGCGCGGCA-3' motif in vitro. |
| Protein Name | Rna-Binding Protein 4Lark HomologHlarkRna-Binding Motif Protein 4Rna-Binding Motif Protein 4a |
| Database Links | Reactome: R-HSA-400253 |
| Cellular Localisation | NucleusNucleolusNucleus SpeckleCytoplasmCytoplasmic GranuleUndergoes Continuous Nucleocytoplasmic ShuttlingUpon Nuclear Import Colocalizes With Sr Proteins In Nuclear SpecklesArsenite Stress-Induced Phosphorylation Increases Its Subcellular Relocalization From The Nucleus To The Cytoplasm And To Cytoplasmic Stress Granules (Sg) Via A P38 Mapk Signaling PathwayPrimarily Localized In Nucleus And Nucleoli Under Cell Growth Conditions And Accumulated In The Cytoplasm And Cytoplasm Perinuclear Granules Upon Muscle Cell Differentiation |
| Alternative Antibody Names | Anti-Rna-Binding Protein 4 antibodyAnti-Lark Homolog antibodyAnti-Hlark antibodyAnti-Rna-Binding Motif Protein 4 antibodyAnti-Rna-Binding Motif Protein 4a antibodyAnti-RBM4 antibodyAnti-RBM4A antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
