| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-RAD51-associated protein 1 (90-170 aa) is suitable for use in Western Blot and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-2000 ELISA 1:5000-20000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | RAD51AP1 |
| Gene ID: | 10635 |
| Uniprot ID: | R51A1_HUMAN |
| Immunogen Region: | 90-170 aa |
| Specificity: | R51A1 Polyclonal Antibody detects endogenous levels of protein. |
| Immunogen: | Synthesized peptide derived from the human protein at the amino acid range 90-170 |
| Post Translational Modifications | Sumoylation with SUMO2/3 by NSMCE2/MMS21 promotes stabilization, possibly by preventing ubiquitination. Sumoylation is required for alternative lengthening of telomeres (ALT) pathway. |
| Function | Structure-specific DNA-binding protein involved in DNA repair by promoting RAD51-mediated homologous recombination. Acts by stimulating D-Loop formation by RAD51: specifically enhances joint molecule formation through its structure-specific DNA interaction and its interaction with RAD51. Binds single-stranded DNA (ssDNA), double-stranded DNA (dsDNA) and secondary DNA structures, such as D-loop structures: has a strong preference for branched-DNA structures that are obligatory intermediates during joint molecule formation. Cooperates with WDR48/UAF1 to stimulate RAD51-mediated homologous recombination: both WDR48/UAF1 and RAD51AP1 have coordinated role in DNA-binding during homologous recombination and DNA repair. WDR48/UAF1 and RAD51AP1 also have a coordinated role in DNA-binding to promote USP1-mediated deubiquitination of FANCD2. Also involved in meiosis by promoting DMC1-mediated homologous meiotic recombination. Key mediator of alternative lengthening of telomeres (ALT) pathway, a homology-directed repair mechanism of telomere elongation that controls proliferation in aggressive cancers, by stimulating homologous recombination. May also bind RNA.additional evidences are however required to confirm RNA-binding in vivo. |
| Protein Name | Rad51-Associated Protein 1 Hsrad51ap1 Rad51-Interacting Protein |
| Database Links | Reactome: R-HSA-5685942 Reactome: R-HSA-5693554 Reactome: R-HSA-5693568 Reactome: R-HSA-5693579 Reactome: R-HSA-9701192 Reactome: R-HSA-9704331 Reactome: R-HSA-9704646 Reactome: R-HSA-9709603 |
| Cellular Localisation | Chromosome Nucleus Telomere Colocalizes With Rad51 To Multiple Nuclear Foci Colocalizes With Dmc1 On Meiotic Chromatin |
| Alternative Antibody Names | Anti-Rad51-Associated Protein 1 antibody Anti-Hsrad51ap1 antibody Anti-Rad51-Interacting Protein antibody Anti-RAD51AP1 antibody Anti-PIR51 antibody |
Information sourced from Uniprot.org