• Western blot analysis of extracts of various cell lines, using PURA antibody (STJ116356) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-PURA antibody (203-322) (STJ116356)

SKU:
STJ116356

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PURA (203-322) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PURA
Gene ID: 5813
Uniprot ID: PURA_HUMAN
Immunogen Region: 203-322
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 203-322 of human PURA (NP_005850.1).
Immunogen Sequence: AKLIDDYGVEEEPAELPEGT SLTVDNKRFFFDVGSNKYGV FMRVSEVKPTYRNSITVPYK VWAKFGHTFCKYSEEMKKIQ EKQREKRAACEQLHQQQQQQ QEETAAATLLLQGEEEGEED
Function This is a probable transcription activator that specifically binds the purine-rich single strand of the PUR element located upstream of the MYC gene. May play a role in the initiation of DNA replication and in recombination.
Protein Name Transcriptional Activator Protein Pur-Alpha
Purine-Rich Single-Stranded Dna-Binding Protein Alpha
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Transcriptional Activator Protein Pur-Alpha antibody
Anti-Purine-Rich Single-Stranded Dna-Binding Protein Alpha antibody
Anti-PURA antibody
Anti-PUR1 antibody

Information sourced from Uniprot.org

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