Western blot analysis of extracts of various cell lines, using PRKAR1B antibody (STJ112164) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PRKAR1B antibody (190-290) (STJ112164)

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STJ112164
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Covered by St John's Laboratory Guarantee

Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:4000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRKAR1B
Gene ID: 5575
Uniprot ID: KAP1_HUMAN
Immunogen Region: 190-290
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 190-290 of human PRKAR1B (NP_002726.1).
Immunogen Sequence: WVTNISEGGSFGELALIYGT PRAATVKAKTDLKLWGIDRD SYRRILMGSTLRKRKMYEEF LSKVSILESLEKWERLTVAD ALEPVQFEDGEKIVVQGEPG D
Tissue Specificity Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
Post Translational Modifications The pseudophosphorylation site binds to the substrate-binding region of the catalytic chain, resulting in the inhibition of its activity.
Function Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells.
Protein Name Camp-Dependent Protein Kinase Type I-Beta Regulatory Subunit
Database Links Reactome: R-HSA-163615
Reactome: R-HSA-164378
Reactome: R-HSA-180024
Reactome: R-HSA-381676
Reactome: R-HSA-432040
Reactome: R-HSA-442720
Reactome: R-HSA-5610787
Reactome: R-HSA-9634597
Reactome: R-HSA-9660821
Reactome: R-HSA-9664323
Reactome: R-HSA-983231
Cellular Localisation Cell Membrane
Alternative Antibody Names Anti-Camp-Dependent Protein Kinase Type I-Beta Regulatory Subunit antibody
Anti-PRKAR1B antibody

Information sourced from Uniprot.org

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