| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PRDM9 |
| Gene ID: |
56979 |
| Uniprot ID: |
PRDM9_HUMAN |
| Immunogen Region: |
413-525 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 413-525 of human PRDM9 (NP_064612.2). |
| Immunogen Sequence: |
SQNFPGPSARKLLQPENPCP GDQNQEQQYPDPHSRNDKTK GQEIKERSKLLNKRTWQREI SRAFSSPPKGQMGSCRVGKR IMEEESRTGQKVNPGNTGKL FVGVGISRIAKVK |
| Post Translational Modifications | Mono-methylated.automethylated. Tri-methylated.automethylated. Mono-methylation is predominant.automethylation is lower and slower than H3 peptide methylation and is in a highest S-adenosyl-L-methionine concentration-dependent. There are two major sites for automethylation at Lys-368 and Lys-374. Lysines can be simultaneously methylated, such as Lys-368(me3)/Lys-372(me1), Lys-368(me1)/Lys-374(me1) and Lys-368(me1)/Lys-372(me1)/Lys-374(me1). Automethylation is an intramolecular (cis) process. |
| Function | Histone methyltransferase that sequentially mono-, di-, and tri-methylates both 'Lys-4' (H3K4) and 'Lys-36' (H3K36) of histone H3 to produce respectively trimethylated 'Lys-4' (H3K4me3) and trimethylated 'Lys-36' (H3K36me3) histone H3 and plays a key role in meiotic prophase by determining hotspot localization thereby promoting meiotic recombination. Can also methylate all four core histones with H3 being the best substrate and the most highly modified. Is also able, on one hand, to mono and di-methylate H4K20 and on other hand to trimethylate H3K9 with the di-methylated H3K9 as the best substrate. During meiotic prophase, binds specific DNA sequences through its zinc finger domains thereby determining hotspot localization where it promotes local H3K4me3 and H3K36me3 enrichment on the same nucleosomes through its histone methyltransferase activity. Thereby promotes double-stranded breaks (DSB) formation, at this subset of PRDM9-binding sites, that initiates meiotic recombination for the proper meiotic progression. During meiotic progression hotspot-bound PRDM9 interacts with several complexes.in early leptonema binds CDYL and EHMT2 followed by EWSR1 and CXXC1 by the end of leptonema. EWSR1 joins PRDM9 with the chromosomal axis through REC8. In this way, controls the DSB repair pathway, pairing of homologous chromosomes and sex body formation. Moreover plays a central role in the transcriptional activation of genes during early meiotic prophase thanks to H3K4me3 and H3K36me3 enrichment that represents a specific tag for epigenetic transcriptional activation. In addition performs automethylation. Acetylation and phosphorylation of histone H3 attenuate or prevent histone H3 methylation. |
| Protein Name | Histone-Lysine N-Methyltransferase Prdm9Pr Domain Zinc Finger Protein 9Pr Domain-Containing Protein 9Protein-Lysine N-Methyltransferase Prdm9Histone H3-Lysine36 N-Trimethyltransferase Prdm9Histone H3-Lysine4 N-Trimethyltransferase Prdm9Histone H3-Lysine9 N-Trimethyltransferase Prdm9Histone H4-N-Methyl-L-Lysine20 N-Methyltransferase Prdm9Histone H4-Lysine20 N-Methyltransferase Prdm9 |
| Database Links | Reactome: R-HSA-3214841Reactome: R-HSA-912446 |
| Cellular Localisation | NucleusChromosomeLocalizes In Nuclei Of Pre-LeptoteneLeptoteneAnd Early To Mid-Zygotene Spermatocytes |
| Alternative Antibody Names | Anti-Histone-Lysine N-Methyltransferase Prdm9 antibodyAnti-Pr Domain Zinc Finger Protein 9 antibodyAnti-Pr Domain-Containing Protein 9 antibodyAnti-Protein-Lysine N-Methyltransferase Prdm9 antibodyAnti-Histone H3-Lysine36 N-Trimethyltransferase Prdm9 antibodyAnti-Histone H3-Lysine4 N-Trimethyltransferase Prdm9 antibodyAnti-Histone H3-Lysine9 N-Trimethyltransferase Prdm9 antibodyAnti-Histone H4-N-Methyl-L-Lysine20 N-Methyltransferase Prdm9 antibodyAnti-Histone H4-Lysine20 N-Methyltransferase Prdm9 antibodyAnti-PRDM9 antibodyAnti-PFM6 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
