Western blot analysis of various lysates using PRDM15 Rabbit pAb (STJ110669) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-PRDM15 antibody (1268-1507) (STJ110669)

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STJ110669
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRDM15
Gene ID: 63977
Uniprot ID: PRD15_HUMAN
Immunogen Region: 1268-1507
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1268-1507 of human PRDM15 (NP_071398.3).
Immunogen Sequence: IDASSIGIVQPELTLEQEDL AEGKHGKAAKRSHKRKQKPE EEAGAPVPEDATFSEYSEKE TEFTGSVGDETNSAVQSIQQ VVVTLGDPNVTTPSSSVGLT NITVTPITTAAATQFTNLQP VAVGHLTTPERQLQLDNSIL TVTFDTVSGSAMLHNRQNDV QIHPQPEASNPQSVAHFINL TTLVNSITPLGSQLSDQHPL TWRAVPQTDVLPPSQPQAPP QQAAQPQVQAEQQQQQMYS
Tissue Specificity Detected in all tissues examined.
Function Sequence-specific DNA-binding transcriptional regulator. Plays a role as a molecular node in a transcriptional network regulating embryonic development and cell fate decision. Stimulates the expression of upstream key transcriptional activators and repressors of the Wnt/beta-catenin and MAPK/ERK pathways, respectively, that are essential for naive pluripotency and self-renewal maintenance of embryonic stem cells (ESCs). Specifically promotes SPRY1 and RSPO1 transcription activation through recognition and direct binding of a specific DNA sequence in their promoter regions. Involved in early embryo development. Also plays a role in induced pluripotent stem cells (iPSCs) reprogramming.
Protein Name Pr Domain Zinc Finger Protein 15
Pr Domain-Containing Protein 15
Zinc Finger Protein 298
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Pr Domain Zinc Finger Protein 15 antibody
Anti-Pr Domain-Containing Protein 15 antibody
Anti-Zinc Finger Protein 298 antibody
Anti-PRDM15 antibody
Anti-C21orf83 antibody
Anti-ZNF298 antibody

Information sourced from Uniprot.org

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