| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-DNA-directed RNA polymerase III subunit RPC7 (151-200 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000 IHC-P 1:100-1:300 ELISA 1:20000 IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | POLR3G |
| Gene ID: | 10622 |
| Uniprot ID: | RPC7_HUMAN |
| Immunogen Region: | 151-200 aa |
| Specificity: | POLR3G Polyclonal Antibody detects endogenous levels of POLR3G protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the Internal region of human POLR3G at the amino acid range 151-200 |
| Function | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III (Pol III) which synthesizes small non-coding RNAs including 5S rRNA, snRNAs, tRNAs and miRNAs from at least 500 distinct genomic loci. Acts as a long tether that bridges POLR3C/RPC3-POLR3F/RPC6-POLR3G/RPC7 heterotrimer and the mobile stalk of Pol III, coordinating the dynamics of Pol III stalk and clamp modules during the transition from apo to elongation state. Pol III exists as two alternative complexes defined by the mutually exclusive incorporation of subunit POLR3G/RPC7alpha or POLR3GL/RPC7beta. POLR3G/RPC7alpha modulates Pol III transcriptome by specifically enhancing the transcription of snaR-A non-coding RNAs. At resting state, occupies the active site of apo Pol III and keeps Pol III in an autoinhibitory mode, preventing non-specific transcription. Pol III plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as a nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs), induce type I interferon and NF-kappa-B through the RIG-I pathway. |
| Protein Name | Dna-Directed Rna Polymerase Iii Subunit Rpc7 Rna Polymerase Iii Subunit C7 Dna-Directed Rna Polymerase Iii Subunit G Rna Polymerase Iii 32 Kda Apha Subunit Rpc32-Alpha Rna Polymerase Iii 32 Kda Subunit Rpc32 |
| Database Links | Reactome: R-HSA-1834949 Reactome: R-HSA-73780 Reactome: R-HSA-73980 Reactome: R-HSA-749476 Reactome: R-HSA-76061 Reactome: R-HSA-76066 Reactome: R-HSA-76071 |
| Cellular Localisation | Nucleus Cytoplasm Excluded From Nucleoli In Zygotes And The 2-Cell Stage Embryos Mainly In The Cytoplasm Starts To Localize To The Nucleus In The 8-16 Cell Stage Embryo And Early Blastocysts |
| Alternative Antibody Names | Anti-Dna-Directed Rna Polymerase Iii Subunit Rpc7 antibody Anti-Rna Polymerase Iii Subunit C7 antibody Anti-Dna-Directed Rna Polymerase Iii Subunit G antibody Anti-Rna Polymerase Iii 32 Kda Apha Subunit antibody Anti-Rpc32-Alpha antibody Anti-Rna Polymerase Iii 32 Kda Subunit antibody Anti-Rpc32 antibody Anti-POLR3G antibody |
Information sourced from Uniprot.org