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Immunohistochemistry analysis of PMS2. Sample: Human colon, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2min. Primary antibody: 1:3000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:200, RT, 1h.
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Immunohistochemistry analysis of PMS2. Sample: Human small intestine, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2min. Primary antibody: 1:3000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:200 RT, 1h.
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Immunohistochemistry analysis of PMS2. Sample: mouse kidney, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2min. Primary antibody: 1:3000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:200 RT, 1h.
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Immunohistochemistry analysis of PMS2. Sample: mouse smal lintestine, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2min. Primary antibody: 1:3000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:200 RT, 1h.
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Immunohistochemistry analysis of PMS2. Sample: mouse testis, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2min. Primary antibody: 1:3000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:200 RT, 1h.
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Immunohistochemistry analysis of PMS2. Sample: Ratcolon, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2min. Primary antibody: 1:3000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:200 RT, 1h.
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Immunohistochemistry analysis of PMS2. Sample: Ratkidney, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2min. Primary antibody: 1:3000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:200 RT, 1h.
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Immunohistochemistry analysis of PMS2. Sample: Rat testis, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2min. Primary antibody: 1:3000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:200 RT, 1h.