• Western blot analysis of extracts of various cell lines, using PKLR antibody (STJ114217) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PKLR antibody (446-556) (STJ114217)

SKU:
STJ114217

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PKLR (446-556) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PKLR
Gene ID: 5313
Uniprot ID: KPYR_HUMAN
Immunogen Region: 446-556
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 446-556 of human PKLR (NP_000289.1).
Immunogen Sequence: PLSRDPTEVTAIGAVEAAFK CCAAAIIVLTTTGRSAQLLS RYRPRAAVIAVTRSAQAARQ VHLCRGVFPLLYREPPEAIW ADDVDRRVQFGIESGKLRGF LRVGDLVIVVT
Function Pyruvate kinase that catalyzes the conversion of phosphoenolpyruvate to pyruvate with the synthesis of ATP, and which plays a key role in glycolysis.
Protein Name Pyruvate Kinase Pklr
Pyruvate Kinase 1
Pyruvate Kinase Isozymes L/R
R-Type/L-Type Pyruvate Kinase
Red Cell/Liver Pyruvate Kinase
Database Links Reactome: R-HSA-163765 P30613-1
Reactome: R-HSA-210745 P30613-2
Reactome: R-HSA-70171
Reactome: R-HSA-9692914 P30613-2
Alternative Antibody Names Anti-Pyruvate Kinase Pklr antibody
Anti-Pyruvate Kinase 1 antibody
Anti-Pyruvate Kinase Isozymes L/R antibody
Anti-R-Type/L-Type Pyruvate Kinase antibody
Anti-Red Cell/Liver Pyruvate Kinase antibody
Anti-PKLR antibody
Anti-PK1 antibody
Anti-PKL antibody

Information sourced from Uniprot.org

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