| Host: | Rabbit |
| Applications: | WB |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Phospho-TRPM7-Ser-1569 is suitable for use in Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Purification: | Antigen Affinity Purified |
| Dilution Range: | WB 1:1000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | TRPM7 |
| Gene ID: | 54822 |
| Uniprot ID: | TRPM7_HUMAN |
| Specificity: | The antibody was cross-adsorbed to unphosphorylated TRPM7 (Ser-1569) then affinity purified using phospho-TRPM7 (Ser-1569) peptide. This antibody detects a 220 kDa* protein on SDS-PAGE immunoblots of human MDA-MB-231 stimulated with calyculin A and r |
| Immunogen: | Phospho-TRPM7 (Ser-1569) synthetic peptide (coupled to carrier) corresponding to amino acids surrounding Ser-1569 in human TRPM7. This site is well conserved in rat and mouse TRPM7, and is conserved in TRPM6 (Thr-1724) however the site is threonine i |
| Function | Bifunctional protein that combines an ion channel with an intrinsic kinase domain, enabling it to modulate cellular functions either by conducting ions through the pore or by phosphorylating downstream proteins via its kinase domain. The channel is highly permeable to divalent cations, specifically calcium (Ca2+), magnesium (Mg2+) and zinc (Zn2+) and mediates their influx. Controls a wide range of biological processes such as Ca2(+), Mg(2+) and Zn(2+) homeostasis, vesicular Zn(2+) release channel and intracellular Ca(2+) signaling, embryonic development, immune responses, cell motility, proliferation and differentiation. The C-terminal alpha-kinase domain autophosphorylates cytoplasmic residues of TRPM7. In vivo, TRPM7 phosphorylates SMAD2, suggesting that TRPM7 kinase may play a role in activating SMAD signaling pathways. In vitro, TRPM7 kinase phosphorylates ANXA1 (annexin A1), myosin II isoforms and a variety of proteins with diverse cellular functions. TRPM7 channel, cleaved form: The cleaved channel exhibits substantially higher current and potentiates Fas receptor signaling. TRPM7 kinase, cleaved form: The C-terminal kinase domain can be cleaved from the channel segment in a cell-type-specific fashion. In immune cells, the TRPM7 kinase domain is clipped from the channel domain by caspases in response to Fas-receptor stimulation. The cleaved kinase fragments can translocate to the nucleus, and bind chromatin-remodeling complex proteins in a Zn(2+)-dependent manner to ultimately phosphorylate specific Ser/Thr residues of histones known to be functionally important for cell differentiation and embryonic development. |
| Protein Name | Transient Receptor Potential Cation Channel Subfamily M Member 7 Channel-Kinase 1 Long Transient Receptor Potential Channel 7 Ltrpc-7 Ltrpc7 Cleaved Into - Trpm7 Kinase - Cleaved Form M7ck - Trpm7 Channel - Cleaved Form |
| Database Links | Reactome: R-HSA-3295583 |
| Cellular Localisation | Cell Membrane Multi-Pass Membrane Protein Cytoplasmic Vesicle Membrane Localized Largely In Intracellular Zn(2+)-Storage Vesicles Trpm7 Kinase Cleaved Form: Nucleus |
| Alternative Antibody Names | Anti-Transient Receptor Potential Cation Channel Subfamily M Member 7 antibody Anti-Channel-Kinase 1 antibody Anti-Long Transient Receptor Potential Channel 7 antibody Anti-Ltrpc-7 antibody Anti-Ltrpc7 Cleaved Into - Trpm7 Kinase - Cleaved Form antibody Anti-M7ck - Trpm7 Channel - Cleaved Form antibody Anti-TRPM7 antibody Anti-CHAK1 antibody Anti-LTRPC7 antibody |
Information sourced from Uniprot.org