• Western blot analysis of lysates from COS7, Jurkat cells, treated or untreated with TNFa, (Green) primary antibody was diluted at 1:1000, 4°C over night, secondary antibody was diluted at 1:10000, 37°C 1hour. (Red) loading contrl antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody was diluted at 1:10000, 37°C 1hour.
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using STAT3 (Phospho-Ser727) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of lysates from COS7 cells, using STAT3 (Phospho-Ser727) Antibody. The lane on the right is blocked with the phospho peptide.
  • Western blot analysis of various cells using Phospho-Stat3 (S727) Polyclonal Antibody diluted at 1：2000
  • Western blot analysis of COS7 cells using Phospho-Stat3 (S727) Polyclonal Antibody diluted at 1：2000
  • Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1, Stat3 (phospho Ser727) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1, Stat3 (phospho Ser727) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue. 1, Stat3 (phospho Ser727) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1, Stat3 (phospho Ser727) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1, Stat3 (phospho Ser727) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1, Stat3 (phospho Ser727) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1, Stat3 (phospho Ser727) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1, Stat3 (phospho Ser727) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunofluorescence analysis of rat-heart tissue. 1, Stat3 (phospho Ser727) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of rat-heart tissue. 1, Stat3 (phospho Ser727) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of Hela cell. 1, Stat3 (phospho Ser727) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Caspase 9 monoclonal antibody (3-20) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Phospho-STAT3-Ser727 antibody (694-743 aa) (STJ90410)

SKU:
STJ90410

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: IF/WB/IHC/IP/ELISA
Reactivity: Human/Mouse/Rat/Monkey
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Signal transducer and activator of transcription 3-Ser727 (694-743 aa) is suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry, Immunoprecipitation and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IF 1:50-200
WB 1:500-1:2000
IHC 1:100-1:300
IP 2-5 ug mg/lysate
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: STAT3
Gene ID: 6774
Uniprot ID: STAT3_HUMAN
Immunogen Region: 694-743 aa
Specificity: Phospho-Stat3 (S727) Polyclonal Antibody detects endogenous levels of Stat3 protein only when phosphorylated at S727.
Immunogen: The antiserum was produced against synthesized peptide derived from the human STAT3 around the phosphorylation site of Ser727 at the amino acid range 694-743
Post Translational Modifications Tyrosine phosphorylated upon stimulation with EGF. Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4. Activated through tyrosine phosphorylation by BMX. Tyrosine phosphorylated in response to IL6, IL11, LIF, CNTF, KITLG/SCF, CSF1, EGF, PDGF, IFN-alpha, LEP and OSM. Activated KIT promotes phosphorylation on tyrosine residues and subsequent translocation to the nucleus. Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Serine phosphorylation is important for the formation of stable DNA-binding STAT3 homodimers and maximal transcriptional activity. ARL2BP may participate in keeping the phosphorylated state of STAT3 within the nucleus. Upon LPS challenge, phosphorylated within the nucleus by IRAK1. Upon erythropoietin treatment, phosphorylated on Ser-727 by RPS6KA5. Phosphorylation at Tyr-705 by PTK6, isoform M2 of PKM (PKM2) or FER leads to an increase of its transcriptional activity. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates IL6/interleukin-6 signaling. Acetylated on lysine residues by CREBBP. Deacetylation by LOXL3 leads to disrupt STAT3 dimerization and inhibit STAT3 transcription activity. Oxidation of lysine residues to allysine on STAT3 preferentially takes place on lysine residues that are acetylated. Some lysine residues are oxidized to allysine by LOXL3, leading to disrupt STAT3 dimerization and inhibit STAT3 transcription activity. Oxidation of lysine residues to allysine on STAT3 preferentially takes place on lysine residues that are acetylated. (Microbial infection) Phosphorylated on Tyr-705 in the presence of S.typhimurium SarA.
Function Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors. Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Upon activation of IL6ST/gp130 signaling by interleukin-6 (IL6), binds to the IL6-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA. Acts as a regulator of inflammatory response by regulating differentiation of naive CD4(+) T-cells into T-helper Th17 or regulatory T-cells (Treg): deacetylation and oxidation of lysine residues by LOXL3, leads to disrupt STAT3 dimerization and inhibit its transcription activity. Involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1. Mediates the effects of LEP on melanocortin production, body energy homeostasis and lactation. May play an apoptotic role by transctivating BIRC5 expression under LEP activation. Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity. Plays a crucial role in basal beta cell functions, such as regulation of insulin secretion.
Protein Name Signal Transducer And Activator Of Transcription 3
Acute-Phase Response Factor
Database Links Reactome: R-HSA-1059683
Reactome: R-HSA-111453
Reactome: R-HSA-1266695
Reactome: R-HSA-1433557
Reactome: R-HSA-1839117
Reactome: R-HSA-186763
Reactome: R-HSA-198745
Reactome: R-HSA-201556
Reactome: R-HSA-2559582
Reactome: R-HSA-2586552
Reactome: R-HSA-2892247
Reactome: R-HSA-390471
Reactome: R-HSA-452723
Reactome: R-HSA-6783783
Reactome: R-HSA-6785807
Reactome: R-HSA-8849474
Reactome: R-HSA-8854691
Reactome: R-HSA-8875791
Reactome: R-HSA-8983432
Reactome: R-HSA-8984722
Reactome: R-HSA-8985947
Reactome: R-HSA-9008059
Reactome: R-HSA-9020933
Reactome: R-HSA-9020956
Reactome: R-HSA-9020958
Reactome: R-HSA-9616222
Reactome: R-HSA-9670439
Reactome: R-HSA-9673767
Reactome: R-HSA-9673770
Reactome: R-HSA-9674555
Reactome: R-HSA-9680350
Reactome: R-HSA-9701898
Reactome: R-HSA-9705462
Reactome: R-HSA-9707564
Reactome: R-HSA-9725370
Reactome: R-HSA-9725371
Reactome: R-HSA-982772
Cellular Localisation Cytoplasm
Nucleus
Shuttles Between The Nucleus And The Cytoplasm
Translocated Into The Nucleus Upon Tyrosine Phosphorylation And Dimerization
In Response To Signaling By Activated Fgfr1
Fgfr2
Fgfr3 Or Fgfr4
Constitutive Nuclear Presence Is Independent Of Tyrosine Phosphorylation
Predominantly Present In The Cytoplasm Without Stimuli
Upon Leukemia Inhibitory Factor (Lif) Stimulation
Accumulates In The Nucleus
The Complex Composed Of Bart And Arl2 Plays An Important Role In The Nuclear Translocation And Retention Of Stat3
Identified In A Complex With Lyn And Pag1
Alternative Antibody Names Anti-Signal Transducer And Activator Of Transcription 3 antibody
Anti-Acute-Phase Response Factor antibody
Anti-STAT3 antibody
Anti-APRF antibody

Information sourced from Uniprot.org

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