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Anti-Phospho-PIK3R1-Tyr607 antibody (573-622 aa) (STJ90925)

Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1, PI 3-kinase p85 Alpha (phospho Tyr607) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1, PI 3-kinase p85 Alpha (phospho Tyr607) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-colon tissue. 1, PI 3-kinase p85 Alpha (phospho Tyr607) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemistry analysis of paraffin-embedded human brain, using PI3-kinase p85-alpha (Phospho-Tyr607) Antibody. The picture on the right is blocked with the phospho peptide.
Immunofluorescence analysis of rat-heart tissue. 1, PI 3-kinase p85 Alpha (phospho Tyr607) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunohistochemical analysis of paraffin-embedded Mouse-spleen tissue. 1, PI 3-kinase p85 Alpha (phospho Tyr607) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Western blot analysis of KB cells using Phospho-PI 3-kinase p85 Alpha (Y607) Polyclonal Antibody diluted at 1：1000
Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1, PI 3-kinase p85 Alpha (phospho Tyr607) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1, PI 3-kinase p85 Alpha (phospho Tyr607) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1, PI 3-kinase p85 Alpha (phospho Tyr607) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1, PI 3-kinase p85 Alpha (phospho Tyr607) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1, PI 3-kinase p85 Alpha (phospho Tyr607) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1, PI 3-kinase p85 Alpha (phospho Tyr607) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1, PI 3-kinase p85 Alpha (phospho Tyr607) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1, PI 3-kinase p85 Alpha (phospho Tyr607) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-spinal-cord tissue. 1, PI 3-kinase p85 Alpha (phospho Tyr607) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue. 1, PI 3-kinase p85 Alpha (phospho Tyr607) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-brain tissue. 1, PI 3-kinase p85 Alpha (phospho Tyr607) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Western blot analysis of various cells using Phospho-PI 3-kinase p85 Alpha (Y607) Polyclonal Antibody diluted at 1：1000
Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1, PI 3-kinase p85 Alpha (phospho Tyr607) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human placenta. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1, PI 3-kinase p85 Alpha (phospho Tyr607) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Western blot analysis of lysates from rat kidney, using PI3-kinase p85-alpha (Phospho-Tyr607) Antibody. The lane on the right is blocked with the phospho peptide.
Immunofluorescence analysis of rat-heart tissue. 1, PI 3-kinase p85 Alpha (phospho Tyr607) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-PIK3R1-Tyr607 antibody (573-622 aa) (STJ90925)

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STJ90925

£46.00 - £226.00

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General Information
Product Properties
Target Information
Additional Info
Warranty Information

Host:	Rabbit
Applications:	IF/WB/IHC/ELISA
Reactivity:	Human/Mouse/Rat/Chicken
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-Phospho-Phosphatidylinositol 3-kinase regulatory subunit alpha-Tyr607 (573-622 aa) is suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry and ELISA research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	IF 1:50-200 WB 1:500-1:2000 IHC 1:100-1:300 ELISA 1:10000
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	PIK3R1
Gene ID:	5295
Uniprot ID:	P85A_HUMAN
Immunogen Region:	573-622 aa
Specificity:	Phospho-PI 3-kinase p85 Alpha (Y607) Polyclonal Antibody detects endogenous levels of PI 3-kinase p85 Alpha protein only when phosphorylated at Y607.
Immunogen:	The antiserum was produced against synthesized peptide derived from the human PI3-kinase p85-alpha around the phosphorylation site of Tyr607 at the amino acid range 573-622

Post Translational Modifications	Polyubiquitinated in T-cells by CBLB.which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation. In adipose tissue, polyubiquitinated by the BCR(KBTBD2) E3 ubiquitin ligase complex.recognized by KBTBD2 through the SH2 domains, undergoes 'Lys-48'-linked polyubiquitination leading to its degradation. Phosphorylated. Tyrosine phosphorylated in response to signaling by FGFR1, FGFR2, FGFR3 and FGFR4. Phosphorylated by CSF1R. Phosphorylated by ERBB4. Phosphorylated on tyrosine residues by TEK/TIE2. Dephosphorylated by PTPRJ. Phosphorylated by PIK3CA at Ser-608.phosphorylation is stimulated by insulin and PDGF. The relevance of phosphorylation by PIK3CA is however unclear. Phosphorylated in response to KIT and KITLG/SCF. Phosphorylated by FGR.
Function	Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling. Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement.
Protein Name	Phosphatidylinositol 3-Kinase Regulatory Subunit Alpha Pi3-Kinase Regulatory Subunit Alpha Pi3k Regulatory Subunit Alpha Ptdins-3-Kinase Regulatory Subunit Alpha Phosphatidylinositol 3-Kinase 85 Kda Regulatory Subunit Alpha Pi3-Kinase Subunit P85-Alpha Ptdins-3-Kinase Regulatory Subunit P85-Alpha
Database Links	Reactome: R-HSA-109704 Reactome: R-HSA-112399 Reactome: R-HSA-114604 Reactome: R-HSA-1236382 Reactome: R-HSA-1250342 Reactome: R-HSA-1257604 Reactome: R-HSA-1266695 Reactome: R-HSA-1433557 Reactome: R-HSA-1660499 Reactome: R-HSA-180292 Reactome: R-HSA-1839117 Reactome: R-HSA-186763 Reactome: R-HSA-1963642 Reactome: R-HSA-198203 Reactome: R-HSA-201556 Reactome: R-HSA-202424 Reactome: R-HSA-2029485 Reactome: R-HSA-210993 Reactome: R-HSA-2219530 Reactome: R-HSA-2424491 Reactome: R-HSA-2730905 Reactome: R-HSA-373753 Reactome: R-HSA-388841 Reactome: R-HSA-389357 Reactome: R-HSA-416476 Reactome: R-HSA-430116 Reactome: R-HSA-4420097 Reactome: R-HSA-512988 Reactome: R-HSA-5637810 Reactome: R-HSA-5654689 Reactome: R-HSA-5654695 Reactome: R-HSA-5654710 Reactome: R-HSA-5654720 Reactome: R-HSA-5655253 Reactome: R-HSA-5655291 Reactome: R-HSA-5655302 Reactome: R-HSA-5655332 Reactome: R-HSA-5673001 Reactome: R-HSA-6785807 Reactome: R-HSA-6811558 Reactome: R-HSA-8851907 Reactome: R-HSA-8853659 Reactome: R-HSA-8980692 Reactome: R-HSA-9009391 Reactome: R-HSA-9013026 Reactome: R-HSA-9013106 Reactome: R-HSA-9013148 Reactome: R-HSA-9013149 Reactome: R-HSA-9013404 Reactome: R-HSA-9013405 Reactome: R-HSA-9013408 Reactome: R-HSA-9013409 Reactome: R-HSA-9013420 Reactome: R-HSA-9013423 Reactome: R-HSA-9013424 Reactome: R-HSA-9027276 Reactome: R-HSA-9028335 Reactome: R-HSA-9035034 Reactome: R-HSA-912526 Reactome: R-HSA-912631 Reactome: R-HSA-9603381 Reactome: R-HSA-9607240 Reactome: R-HSA-9664565 Reactome: R-HSA-9665348 Reactome: R-HSA-9670439 Reactome: R-HSA-9673767 Reactome: R-HSA-9673770 Reactome: R-HSA-9680350 Reactome: R-HSA-9696264 Reactome: R-HSA-9696270 Reactome: R-HSA-9696273 Reactome: R-HSA-9703465 Reactome: R-HSA-9703648 Reactome: R-HSA-9725370 Reactome: R-HSA-983695
Alternative Antibody Names	Anti-Phosphatidylinositol 3-Kinase Regulatory Subunit Alpha antibody Anti-Pi3-Kinase Regulatory Subunit Alpha antibody Anti-Pi3k Regulatory Subunit Alpha antibody Anti-Ptdins-3-Kinase Regulatory Subunit Alpha antibody Anti-Phosphatidylinositol 3-Kinase 85 Kda Regulatory Subunit Alpha antibody Anti-Pi3-Kinase Subunit P85-Alpha antibody Anti-Ptdins-3-Kinase Regulatory Subunit P85-Alpha antibody Anti-PIK3R1 antibody Anti-GRB1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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