| Host: | Rabbit |
| Applications: | WB |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Phospho-Pancreas/duodenum homeobox protein 1-Ser61 is suitable for use in Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-2000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PDX1 |
| Gene ID: | 3651 |
| Uniprot ID: | PDX1_HUMAN |
| Specificity: | This antibody detects endogenous levels of PDX1 (Phospho-Ser61) at Human, Mouse, Rat |
| Immunogen: | Synthesized peptide derived from human PDX1 (Phospho-Ser61) |
| Tissue Specificity | Duodenum and pancreas (Langerhans islet beta cells and small subsets of endocrine non-beta-cells, at low levels in acinar cells). |
| Post Translational Modifications | Phosphorylated by the SAPK2 pathway at high intracellular glucose concentration. Phosphorylated by HIPK2 on Ser-268 upon glucose accumulation. This phosphorylation mediates subnuclear localization shifting. Phosphorylation by PASK may lead to translocation into the cytosol. |
| Function | Activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. As part of a PDX1:PBX1b:MEIS2b complex in pancreatic acinar cells is involved in the transcriptional activation of the ELA1 enhancer.the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element. Binds preferentially the DNA motif 5'-CTTAATTG-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell. |
| Protein Name | Pancreas/Duodenum Homeobox Protein 1 Pdx-1 Glucose-Sensitive Factor Gsf Insulin Promoter Factor 1 Ipf-1 Insulin Upstream Factor 1 Iuf-1 Islet/Duodenum Homeobox-1 Idx-1 Somatostatin-Transactivating Factor 1 Stf-1 |
| Database Links | Reactome: R-HSA-210745 Reactome: R-HSA-210747 |
| Cellular Localisation | Nucleus Cytoplasm Cytosol |
| Alternative Antibody Names | Anti-Pancreas/Duodenum Homeobox Protein 1 antibody Anti-Pdx-1 antibody Anti-Glucose-Sensitive Factor antibody Anti-Gsf antibody Anti-Insulin Promoter Factor 1 antibody Anti-Ipf-1 antibody Anti-Insulin Upstream Factor 1 antibody Anti-Iuf-1 antibody Anti-Islet/Duodenum Homeobox-1 antibody Anti-Idx-1 antibody Anti-Somatostatin-Transactivating Factor 1 antibody Anti-Stf-1 antibody Anti-PDX1 antibody Anti-IPF1 antibody Anti-STF1 antibody |
Information sourced from Uniprot.org