| Host: |
Rabbit |
| Applications: |
WB/IHC/IF/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-Dual specificity mitogen-activated protein kinase kinase 1 and Dual specificity mitogen-activated protein kinase kinase 2-Ser222/226 (193-242 aa) is suitable for use in Western Blot, Immunohistochemistry, Immun |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-1:2000IHC 1:100-1:300ELISA 1:20000IF 1:50-200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
MAP2K1MAP2K2 |
| Gene ID: |
56045605 |
| Uniprot ID: |
MP2K1_HUMANMP2K2_HUMAN |
| Immunogen Region: |
193-242 aa |
| Specificity: |
Phospho-MEK-1/2 (S222/226) Polyclonal Antibody detects endogenous levels of MEK-1/2 protein only when phosphorylated at S222/226. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from the human MEK1/2 around the phosphorylation site of Ser221 at the amino acid range 193-242 |
| Post Translational Modifications | Phosphorylation at Ser-218 and Ser-222 by MAP kinase kinase kinases (BRAF or MEKK1) positively regulates kinase activity. Also phosphorylated at Thr-292 by MAPK1/ERK2 and at Ser-298 by PAK. MAPK1/ERK2 phosphorylation of Thr-292 occurs in response to cellular adhesion and leads to inhibition of Ser-298 phosphorylation by PAK. Autophosphorylated at Ser-218 and Ser-222, autophosphosphorylation is promoted by NEK10 following UV irradiation. (Microbial infection) Acetylation by Yersinia YopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway. |
| Function | Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Activates BRAF in a KSR1 or KSR2-dependent manner.by binding to KSR1 or KSR2 releases the inhibitory intramolecular interaction between KSR1 or KSR2 protein kinase and N-terminal domains which promotes KSR1 or KSR2-BRAF dimerization and BRAF activation. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis. |
| Protein Name | Dual Specificity Mitogen-Activated Protein Kinase Kinase 1Map Kinase Kinase 1Mapkk 1Mkk1Erk Activator Kinase 1Mapk/Erk Kinase 1Mek 1 |
| Database Links | Reactome: R-HSA-110056Reactome: R-HSA-170968Reactome: R-HSA-445144Reactome: R-HSA-5210891Reactome: R-HSA-5673000Reactome: R-HSA-5674135Reactome: R-HSA-5674499Reactome: R-HSA-5684264Reactome: R-HSA-6802946Reactome: R-HSA-6802948Reactome: R-HSA-6802952Reactome: R-HSA-6802955Reactome: R-HSA-9649948Reactome: R-HSA-9652169Reactome: R-HSA-9656223 |
| Cellular Localisation | CytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeSpindle Pole BodyNucleusMembranePeripheral Membrane ProteinLocalizes At Centrosomes During PrometaphaseMidzone During Anaphase And Midbody During Telophase/CytokinesisMembrane Localization Is Probably Regulated By Its Interaction With Ksr1 |
| Alternative Antibody Names | Anti-Dual Specificity Mitogen-Activated Protein Kinase Kinase 1 antibodyAnti-Map Kinase Kinase 1 antibodyAnti-Mapkk 1 antibodyAnti-Mkk1 antibodyAnti-Erk Activator Kinase 1 antibodyAnti-Mapk/Erk Kinase 1 antibodyAnti-Mek 1 antibodyAnti-MAP2K1 antibodyAnti-MEK1 antibodyAnti-PRKMK1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
