| Host: | Rabbit |
| Applications: | IF/WB/IHC/ELISA |
| Reactivity: | Human/Mouse/Rat/Chicken/Pig/Fish |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Phospho-Mitogen-activated protein kinase 8 and Mitogen-activated protein kinase 9 and Mitogen-activated protein kinase 10-Thr183/Y185 (151-200 aa) is suitable for use in Immunofluorescence, Western Blot, Immunohistoche |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | IF 1:50-200 WB 1:500-2000 IHC 1:50-300 IHC 1:50-300 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MAPK9 MAPK10 5601 5602 5599 MK09_HUMAN MK10_HUMAN MK08_HUMAN |
| Immunogen Region: | 151-200 aa |
| Specificity: | Phospho-JNK1/2/3 (T183/Y185) Polyclonal Antibody detects endogenous levels of JNK1/2/3 protein only when phosphorylated at T183/Y185. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human JNK1/2/3 around the phosphorylation site of Thr183 and Tyr185 at the amino acid range 151-200 |
| Function | Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation. Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy. Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons. In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone. Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the regulation of the circadian clock. Phosphorylates the heat shock transcription factor HSF1, suppressing HSF1-induced transcriptional activity. Phosphorylates POU5F1, which results in the inhibition of POU5F1's transcriptional activity and enhances its proteosomal degradation. Phosphorylates JUND and this phosphorylation is inhibited in the presence of MEN1. In neurons, phosphorylates SYT4 which captures neuronal dense core vesicles at synapses. Phosphorylates EIF4ENIF1/4-ET in response to oxidative stress, promoting P-body assembly. JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. |
| Protein Name | Mitogen-Activated Protein Kinase 8 Map Kinase 8 Mapk 8 Jnk-46 Stress-Activated Protein Kinase 1c Sapk1c Stress-Activated Protein Kinase Jnk1 C-Jun N-Terminal Kinase 1 |
| Database Links | Reactome: R-HSA-111446 Reactome: R-HSA-139910 Reactome: R-HSA-193648 Reactome: R-HSA-205043 Reactome: R-HSA-2559580 Reactome: R-HSA-2871796 Reactome: R-HSA-376172 Reactome: R-HSA-450321 Reactome: R-HSA-450341 Reactome: R-HSA-5693565 Reactome: R-HSA-9007892 Reactome: R-HSA-9673324 |
| Cellular Localisation | Cytoplasm Nucleus Cell Junction Synapse In The Cortical Neurons Predominantly Cytoplasmic And Associated With The Golgi Apparatus And Endosomal Fraction Increased Neuronal Activity Increases Phosphorylated Form At Synapses Colocalizes With Pou5f1 In The Nucleus |
| Alternative Antibody Names | Anti-Mitogen-Activated Protein Kinase 8 antibody Anti-Map Kinase 8 antibody Anti-Mapk 8 antibody Anti-Jnk-46 antibody Anti-Stress-Activated Protein Kinase 1c antibody Anti-Sapk1c antibody Anti-Stress-Activated Protein Kinase Jnk1 antibody Anti-C-Jun N-Terminal Kinase 1 antibody Anti-MAPK8 antibody Anti-JNK1 antibody Anti-PRKM8 antibody Anti-SAPK1 antibody Anti-SAPK1C antibody |
Information sourced from Uniprot.org