| Host: |
Rabbit |
| Applications: |
WB/IF/ELISA |
| Reactivity: |
Human/Rat/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-Heat shock factor protein 1-Ser307 (273-322 aa) is suitable for use in Western Blot, Immunofluorescence and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-1:2000IF 1:200-1:1000ELISA 1:5000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
HSF1 |
| Gene ID: |
3297 |
| Uniprot ID: |
HSF1_HUMAN |
| Immunogen Region: |
273-322 aa |
| Specificity: |
Phospho-HSF1 (S307) Polyclonal Antibody detects endogenous levels of HSF1 protein only when phosphorylated at S307. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from the human HSF1 around the phosphorylation site of Ser307 at the amino acid range 273-322 |
| Function | Functions as a stress-inducible and DNA-binding transcription factor that plays a central role in the transcriptional activation of the heat shock response (HSR), leading to the expression of a large class of molecular chaperones, heat shock proteins (HSPs), that protect cells from cellular insult damage. In unstressed cells, is present in a HSP90-containing multichaperone complex that maintains it in a non-DNA-binding inactivated monomeric form. Upon exposure to heat and other stress stimuli, undergoes homotrimerization and activates HSP gene transcription through binding to site-specific heat shock elements (HSEs) present in the promoter regions of HSP genes. Upon heat shock stress, forms a chromatin-associated complex with TTC5/STRAP and p300/EP300 to stimulate HSR transcription, therefore increasing cell survival. Activation is reversible, and during the attenuation and recovery phase period of the HSR, returns to its unactivated form. Binds to inverted 5'-NGAAN-3' pentamer DNA sequences. Binds to chromatin at heat shock gene promoters. Activates transcription of transcription factor FOXR1 which in turn activates transcription of the heat shock chaperones HSPA1A and HSPA6 and the antioxidant NADPH-dependent reductase DHRS2. Also serves several other functions independently of its transcriptional activity. Involved in the repression of Ras-induced transcriptional activation of the c-fos gene in heat-stressed cells. Positively regulates pre-mRNA 3'-end processing and polyadenylation of HSP70 mRNA upon heat-stressed cells in a symplekin (SYMPK)-dependent manner. Plays a role in nuclear export of stress-induced HSP70 mRNA. Plays a role in the regulation of mitotic progression. Also plays a role as a negative regulator of non-homologous end joining (NHEJ) repair activity in a DNA damage-dependent manner. Involved in stress-induced cancer cell proliferation in a IER5-dependent manner. (Microbial infection) Plays a role in latent human immunodeficiency virus (HIV-1) transcriptional reactivation. Binds to the HIV-1 long terminal repeat promoter (LTR) to reactivate viral transcription by recruiting cellular transcriptional elongation factors, such as CDK9, CCNT1 and EP300. |
| Protein Name | Heat Shock Factor Protein 1Hsf 1Heat Shock Transcription Factor 1Hstf 1 |
| Database Links | Reactome: R-HSA-3371453Reactome: R-HSA-3371511Reactome: R-HSA-3371568Reactome: R-HSA-3371571Reactome: R-HSA-9646399 |
| Cellular Localisation | NucleusCytoplasmNucleoplasmPerinuclear RegionCytoskeletonSpindle PoleMicrotubule Organizing CenterCentrosomeChromosomeCentromereKinetochoreThe Monomeric Form Is Cytoplasmic In Unstressed CellsPredominantly Nuclear Protein In Both Unstressed And Heat Shocked CellsTranslocates In The Nucleus Upon Heat ShockNucleocytoplasmic Shuttling ProteinColocalizes With Ier5 In The NucleusColocalizes With Bag3 To The Nucleus Upon Heat StressLocalizes In Subnuclear Granules Called Nuclear Stress Bodies (Nsbs) Upon Heat ShockColocalizes With Sympk And Sumo1 In Nsbs Upon Heat ShockColocalizes With Prkaca/Pka In The Nucleus And Nsbs Upon Heat ShockRelocalizes From The Nucleus To The Cytoplasm During The Attenuation And Recovery Phase Period Of The Heat Shock ResponseTranslocates In The Cytoplasm In A Ywhae- And Xpo1/Crm1-Dependent MannerTogether With Histone H2axRedistributed In Discrete Nuclear Dna Damage-Induced Foci After Ionizing Radiation (Ir)Colocalizes With Calcium-Responsive Transactivator Ss18l1 At Kinetochore Region On The Mitotic ChromosomesColocalizes With Gamma Tubulin At CentrosomeLocalizes At Spindle Pole In MetaphaseColocalizes With Plk1 At Spindle Poles During Prometaphase |
| Alternative Antibody Names | Anti-Heat Shock Factor Protein 1 antibodyAnti-Hsf 1 antibodyAnti-Heat Shock Transcription Factor 1 antibodyAnti-Hstf 1 antibodyAnti-HSF1 antibodyAnti-HSTF1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
