| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Phospho-Histone deacetylase 3-Ser424 (379-428 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000 IHC 1:100-1:300 IF 1:200-1:1000 ELISA 1:10000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | HDAC3 |
| Gene ID: | 8841 |
| Uniprot ID: | HDAC3_HUMAN |
| Immunogen Region: | 379-428 aa |
| Specificity: | Phospho-HDAC3 (S424) Polyclonal Antibody detects endogenous levels of HDAC3 protein only when phosphorylated at S424. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human HDAC3 around the phosphorylation site of Ser424 at the amino acid range 379-428 |
| Post Translational Modifications | Sumoylated in vitro. Deubiquitinated on 'Lys-63'-linked ubiquitin chains by USP38.leading to a decreased level of histone acetylation. |
| Function | Histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), and some other non-histone substrates. Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes, such as N-Cor repressor complex, which activate the histone deacetylase activity. Participates in the BCL6 transcriptional repressor activity by deacetylating the H3 'Lys-27' (H3K27) on enhancer elements, antagonizing EP300 acetyltransferase activity and repressing proximal gene expression. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation. Contributes, together with XBP1 isoform 1, to the activation of NFE2L2-mediated HMOX1 transcription factor gene expression in a PI(3)K/mTORC2/Akt-dependent signaling pathway leading to endothelial cell (EC) survival under disturbed flow/oxidative stress. Regulates both the transcriptional activation and repression phases of the circadian clock in a deacetylase activity-independent manner. During the activation phase, promotes the accumulation of ubiquitinated BMAL1 at the E-boxes and during the repression phase, blocks FBXL3-mediated CRY1/2 ubiquitination and promotes the interaction of CRY1 and BMAL1. The NCOR1-HDAC3 complex regulates the circadian expression of the core clock gene BMAL1 and the genes involved in lipid metabolism in the liver. Also functions as a deacetylase for non-histone targets, such as KAT5, MEF2D, MAPK14, RARA and STAT3. Serves as a corepressor of RARA, mediating its deacetylation and repression, leading to inhibition of RARE DNA element binding. In association with RARA, plays a role in the repression of microRNA-10a and thereby in the inflammatory response. In addition to protein deacetylase activity, also acts as a protein-lysine deacylase by recognizing other acyl groups: catalyzes removal of (2E)-butenoyl (crotonyl), lactoyl (lactyl) and 2-hydroxyisobutanoyl (2-hydroxyisobutyryl) acyl groups from lysine residues, leading to protein decrotonylation, delactylation and de-2-hydroxyisobutyrylation, respectively. Catalyzes decrotonylation of MAPRE1/EB1. Mediates delactylation NBN/NBS1, thereby inhibiting DNA double-strand breaks (DSBs) via homologous recombination (HR). |
| Protein Name | Histone Deacetylase 3 Hd3 Protein Deacetylase Hdac3 Protein Deacylase Hdac3 Rpd3-2 Smap45 |
| Database Links | Reactome: R-HSA-1368071 Reactome: R-HSA-193670 Reactome: R-HSA-1989781 Reactome: R-HSA-2122947 Reactome: R-HSA-2151201 Reactome: R-HSA-2644606 Reactome: R-HSA-2894862 Reactome: R-HSA-3214815 Reactome: R-HSA-350054 Reactome: R-HSA-381340 Reactome: R-HSA-390471 Reactome: R-HSA-400206 Reactome: R-HSA-400253 Reactome: R-HSA-5617472 Reactome: R-HSA-8940973 Reactome: R-HSA-8943724 Reactome: R-HSA-9022537 Reactome: R-HSA-9022692 Reactome: R-HSA-9029569 Reactome: R-HSA-9609690 Reactome: R-HSA-9701898 Reactome: R-HSA-9707564 Reactome: R-HSA-9707616 |
| Cellular Localisation | Nucleus Chromosome Cytoplasm Cytosol Colocalizes With Xbp1 And Akt1 In The Cytoplasm Predominantly Expressed In The Nucleus In The Presence Of Ccar2 |
| Alternative Antibody Names | Anti-Histone Deacetylase 3 antibody Anti-Hd3 antibody Anti-Protein Deacetylase Hdac3 antibody Anti-Protein Deacylase Hdac3 antibody Anti-Rpd3-2 antibody Anti-Smap45 antibody Anti-HDAC3 antibody |
Information sourced from Uniprot.org