Anti-Phospho-Estrogen Receptor alpha-S118 antibody [ARC1549] (STJ11102593)

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STJ11102593

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Host: Rabbit
Applications: WB
Reactivity: Human, Mouse
Short Description: Rabbit monoclonal anti-Phospho-Estrogen Receptor alpha-S118 antibody is suitable for use in Western Blot.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Monoclonal
Clone ID: ARC1549
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:500-1:2000
Uniprot ID: ESR1_HUMAN
Gene ID: 2099
Gene Symbol: ESR1
Immunogen: A phospho specific peptide corresponding to residues surrounding S118 of human Estrogen Receptor alpha
Tissue Specificity Widely expressed. Isoform 3 is not expressed in the pituitary gland.
Post Translational Modifications Phosphorylated by cyclin A/CDK2 and CK1. Phosphorylation probably enhances transcriptional activity. Self-association induces phosphorylation. Dephosphorylation at Ser-118 by PPP5C inhibits its transactivation activity. Phosphorylated by LMTK3 in vitro. Glycosylated.contains N-acetylglucosamine, probably O-linked. Ubiquitinated.regulated by LATS1 via DCAF1 it leads to ESR1 proteasomal degradation. Deubiquitinated by OTUB1. Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization. Demethylated by JMJD6 at Arg-260. Palmitoylated (isoform 3). Not biotinylated (isoform 3). Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation, but not for signaling mediated by the nuclear hormone receptor.
Function Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements.the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Isoform 3 is involved in activation of NOS3 and endothelial nitric oxide production. Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full-length receptor. Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3. Isoform 3 can bind to ERE and inhibit isoform 1.
Protein Name Estrogen Receptor
Er
Er-Alpha
Estradiol Receptor
Nuclear Receptor Subfamily 3 Group A Member 1
Database Links Reactome: R-HSA-1251985
Reactome: R-HSA-1257604
Reactome: R-HSA-2219530
Reactome: R-HSA-383280
Reactome: R-HSA-4090294
Reactome: R-HSA-5689896
Reactome: R-HSA-6811558
Reactome: R-HSA-8866910
Reactome: R-HSA-8931987
Reactome: R-HSA-8939211
Reactome: R-HSA-8939256
Reactome: R-HSA-8939902
Reactome: R-HSA-9009391
Reactome: R-HSA-9018519
Cellular Localisation Isoform 1: Nucleus
Cytoplasm
Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
A Minor Fraction Is Associated With The Inner Membrane
Isoform 3: Nucleus
Single-Pass Type I Membrane Protein
Associated With The Inner Membrane Via Palmitoylation (Probable)
At Least A Subset Exists As A Transmembrane Protein With A N-Terminal Extracellular Domain
Nucleus
Golgi Apparatus
Colocalizes With Zdhhc7 And Zdhhc21 In The Golgi Apparatus Where Most Probably Palmitoylation Occurs
Associated With The Plasma Membrane When Palmitoylated
Alternative Antibody Names Anti-Estrogen Receptor antibody
Anti-Er antibody
Anti-Er-Alpha antibody
Anti-Estradiol Receptor antibody
Anti-Nuclear Receptor Subfamily 3 Group A Member 1 antibody
Anti-ESR1 antibody
Anti-ESR antibody
Anti-NR3A1 antibody

Information sourced from Uniprot.org

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