• Western blot analysis of extracts of HeLa cells, using Phospho-eIF4E-S209 antibody (STJ11105791) at 1:1000 dilution. HeLa cells were treated by 20% FBS at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-Phospho-EIF4E-S209 antibody [S5791RM] (STJ11105791)

SKU:
STJ11105791

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Host: Rabbit
Applications: WB
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Phospho-Eukaryotic Translation Initiation Factor 4e-S209 is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S5791RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EIF4E
Gene ID: 1977
Uniprot ID: IF4E_HUMAN
Immunogen: A phospho specific peptide corresponding to residues surrounding S209 of human Phospho-eIF4E-S209 (NP_001959.1).
Post Translational Modifications Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex. Phosphorylation also enhances its mRNA transport function. Phosphorylation at Ser-209 is not essential for protein synthesis.
Function Acts in the cytoplasm to initiate and regulate protein synthesis and is required in the nucleus for export of a subset of mRNAs from the nucleus to the cytoplasm which promotes processes such as RNA capping, processing and splicing. Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. This protein recognizes and binds the 7-methylguanosine (m7G)-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Together with EIF4G1, antagonizes the scanning promoted by EIF1-EIF4G1 and is required for TISU translation, a process where the TISU element recognition makes scanning unnecessary. In addition to its role in translation initiation, also acts as a regulator of translation and stability in the cytoplasm. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression: in the complex, EIF4E mediates the binding to the mRNA cap. Component of a multiprotein complex that sequesters and represses translation of proneurogenic factors during neurogenesis. In P-bodies, component of a complex that mediates the storage of translationally inactive mRNAs in the cytoplasm and prevents their degradation. May play an important role in spermatogenesis through translational regulation of stage-specific mRNAs during germ cell development. As well as its roles in translation, also involved in mRNA nucleocytoplasmic transport. Its role in mRNA export from the nucleus to the cytoplasm relies on its ability to bind the m7G cap of RNAs and on the presence of the 50-nucleotide EIF4E sensitivity element (4ESE) in the 3'UTR of sensitive transcripts. Interaction with the 4ESE is mediated by LRPPRC which binds simultaneously to both EIF4E and the 4ESE, thereby acting as a platform for assembly for the RNA export complex. EIF4E-dependent mRNA export is independent of ongoing protein or RNA synthesis and is also NFX1-independent but is XPO1-dependent with LRPPRC interacting with XPO1 to form an EIF4E-dependent mRNA export complex. Alters the composition of the cytoplasmic face of the nuclear pore to promote RNA export by reducing RANBP2 expression, relocalizing nucleoporin NUP214 and increasing expression of RANBP1 and RNA export factors DDX19 and GLE1. Promotes the nuclear export of cyclin CCND1 mRNA. Promotes the nuclear export of NOS2/iNOS mRNA. Promotes the nuclear export of MDM2 mRNA. Promotes the export of additional mRNAs, including others involved in the cell cycle. In the nucleus, binds to capped splice factor-encoding mRNAs and stimulates their nuclear export to enhance splice factor production by increasing their cytoplasmic availability to the translation machinery. May also regulate splicing through interaction with the spliceosome in an RNA and m7G cap-dependent manner. Also binds to some pre-mRNAs and may play a role in their recruitment to the spliceosome. Promotes steady-state capping of a subset of coding and non-coding RNAs by mediating nuclear export of capping machinery mRNAs including RNMT, RNGTT and RAMAC to enhance their translation. Stimulates mRNA 3'-end processing by promoting the expression of several core cleavage complex factors required for mRNA cleavage and polyadenylation, and may also have a direct effect through its interaction with the CPSF3 cleavage enzyme. Rescues cells from apoptosis by promoting activation of serine/threonine-protein kinase AKT1 through mRNA export of NBS1 which potentiates AKT1 phosphorylation and also through mRNA export of AKT1 effectors, allowing for increased production of these proteins.
Protein Name Eukaryotic Translation Initiation Factor 4e
Eif-4e
Eif4e
Eif-4f 25 Kda Subunit
Mrna Cap-Binding Protein
Database Links Reactome: R-HSA-1169408
Reactome: R-HSA-156827
Reactome: R-HSA-159227
Reactome: R-HSA-159230
Reactome: R-HSA-159231
Reactome: R-HSA-166208
Reactome: R-HSA-429947
Reactome: R-HSA-72649
Reactome: R-HSA-72662
Reactome: R-HSA-72702
Reactome: R-HSA-72706
Cellular Localisation Cytoplasm
P-Body
Stress Granule
Nucleus
Nucleus Speckle
Nuclear Body
Interaction With Eif4enif1/4e-T Is Required For Localization To Processing Bodies (P-Bodies)
Imported In The Nucleus Via Interaction With Eif4enif1/4e-T Via A Piggy-Back Mechanism
Sequestered In The Nucleus By Eif4ebp1 And Eif4ebp2
Alternative Antibody Names Anti-Eukaryotic Translation Initiation Factor 4e antibody
Anti-Eif-4e antibody
Anti-Eif4e antibody
Anti-Eif-4f 25 Kda Subunit antibody
Anti-Mrna Cap-Binding Protein antibody
Anti-EIF4E antibody
Anti-EIF4EL1 antibody
Anti-EIF4F antibody

Information sourced from Uniprot.org

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