Anti-Phospho-EIF2AK2-T446 antibody [ARC0293] (STJ11102631)


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Host: Rabbit
Applications: WB
Reactivity: Human
Short Description: Rabbit monoclonal anti-Phospho-EIF2AK2-T446 antibody is suitable for use in Western Blot.
Clonality: Monoclonal
Clone ID: ARC0293
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:500-1:2000
Uniprot ID: E2AK2_HUMAN
Gene ID: 5610
Gene Symbol: EIF2AK2
Immunogen: A phospho specific peptide corresponding to residues surrounding T446 of human EIF2AK2
Tissue Specificity Highly expressed in thymus, spleen and bone marrow compared to non-hematopoietic tissues such as small intestine, liver, or kidney tissues. Colocalizes with GSK3B and TAU in the Alzheimer disease (AD) brain. Elevated levels seen in breast and colon carcinomas, and which correlates with tumor progression and invasiveness or risk of progression.
Post Translational Modifications Autophosphorylated on several Ser, Thr and Tyr residues. Autophosphorylation of Thr-451 is dependent on Thr-446 and is stimulated by dsRNA binding and dimerization. Autophosphorylation apparently leads to the activation of the kinase. Tyrosine autophosphorylation is essential for efficient dsRNA-binding, dimerization, and kinase activation.
Function IFN-induced dsRNA-dependent serine/threonine-protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) and plays a key role in the innate immune response to viral infection. Inhibits viral replication via the integrated stress response (ISR): EIF2S1/eIF-2-alpha phosphorylation in response to viral infection converts EIF2S1/eIF-2-alpha in a global protein synthesis inhibitor, resulting to a shutdown of cellular and viral protein synthesis, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activator ATF4. Exerts its antiviral activity on a wide range of DNA and RNA viruses including hepatitis C virus (HCV), hepatitis B virus (HBV), measles virus (MV) and herpes simplex virus 1 (HHV-1). Also involved in the regulation of signal transduction, apoptosis, cell proliferation and differentiation: phosphorylates other substrates including p53/TP53, PPP2R5A, DHX9, ILF3, IRS1 and the HHV-1 viral protein US11. In addition to serine/threonine-protein kinase activity, also has tyrosine-protein kinase activity and phosphorylates CDK1 at 'Tyr-4' upon DNA damage, facilitating its ubiquitination and proteosomal degradation. Either as an adapter protein and/or via its kinase activity, can regulate various signaling pathways (p38 MAP kinase, NF-kappa-B and insulin signaling pathways) and transcription factors (JUN, STAT1, STAT3, IRF1, ATF3) involved in the expression of genes encoding proinflammatory cytokines and IFNs. Activates the NF-kappa-B pathway via interaction with IKBKB and TRAF family of proteins and activates the p38 MAP kinase pathway via interaction with MAP2K6. Can act as both a positive and negative regulator of the insulin signaling pathway (ISP). Negatively regulates ISP by inducing the inhibitory phosphorylation of insulin receptor substrate 1 (IRS1) at 'Ser-312' and positively regulates ISP via phosphorylation of PPP2R5A which activates FOXO1, which in turn up-regulates the expression of insulin receptor substrate 2 (IRS2). Can regulate NLRP3 inflammasome assembly and the activation of NLRP3, NLRP1, AIM2 and NLRC4 inflammasomes. Plays a role in the regulation of the cytoskeleton by binding to gelsolin (GSN), sequestering the protein in an inactive conformation away from actin.
Protein Name Interferon-Induced - Double-Stranded Rna-Activated Protein Kinase
Eukaryotic Translation Initiation Factor 2-Alpha Kinase 2
Eif-2a Protein Kinase 2
Interferon-Inducible Rna-Dependent Protein Kinase
P1/Eif-2a Protein Kinase
Protein Kinase Rna-Activated
Protein Kinase R
Tyrosine-Protein Kinase Eif2ak2
P68 Kinase
Database Links Reactome: R-HSA-1169408
Reactome: R-HSA-169131
Cellular Localisation Cytoplasm
Perinuclear Region
Nuclear Localization Is Elevated In Acute Leukemia
Myelodysplastic Syndrome (Mds)
Prostate And Lung Cancer Patient Samples Or Cell Lines As Well As Neurocytes From Advanced Creutzfeldt-Jakob Disease Patients
Alternative Antibody Names Anti-Interferon-Induced - Double-Stranded Rna-Activated Protein Kinase antibody
Anti-Eukaryotic Translation Initiation Factor 2-Alpha Kinase 2 antibody
Anti-Eif-2a Protein Kinase 2 antibody
Anti-Interferon-Inducible Rna-Dependent Protein Kinase antibody
Anti-P1/Eif-2a Protein Kinase antibody
Anti-Protein Kinase Rna-Activated antibody
Anti-Pkr antibody
Anti-Protein Kinase R antibody
Anti-Tyrosine-Protein Kinase Eif2ak2 antibody
Anti-P68 Kinase antibody
Anti-EIF2AK2 antibody
Anti-PKR antibody
Anti-PRKR antibody

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