Anti-Phospho-Beta-Catenin-S33/S37 antibody [ARC1523] (STJ11102571)

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STJ11102571

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Host: Rabbit
Applications: WB
Reactivity: Human
Short Description: Rabbit monoclonal anti-Phospho-Beta-Catenin-S33/S37 antibody is suitable for use in Western Blot.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Monoclonal
Clone ID: ARC1523
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:500-1:2000
Uniprot ID: CTNB1_HUMAN
Gene ID: 1499
Gene Symbol: CTNNB1
Immunogen: A phospho specific peptide corresponding to residues surrounding S33/S37 of human beta Catenin
Tissue Specificity Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon. Present in cortical neurons (at protein level). Expressed in breast cancer tissues (at protein level).
Post Translational Modifications Phosphorylation at Ser-552 by AMPK promotes stabilizion of the protein, enhancing TCF/LEF-mediated transcription. Phosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr-41 to Ser-37 and Ser-33. Phosphorylated by NEK2. EGF stimulates tyrosine phosphorylation. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding. Phosphorylated on Ser-33 and Ser-37 by HIPK2 and GSK3B, this phosphorylation triggers proteasomal degradation. Phosphorylation on Ser-191 and Ser-246 by CDK5. Phosphorylation by CDK2 regulates insulin internalization. Phosphorylation by PTK6 at Tyr-64, Tyr-142, Tyr-331 and/or Tyr-333 with the predominant site at Tyr-64 is not essential for inhibition of transcriptional activity. Ubiquitinated by the SCF(BTRC) E3 ligase complex when phosphorylated by GSK3B, leading to its degradation. Ubiquitinated by a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X, leading to its subsequent proteasomal degradation. Ubiquitinated and degraded following interaction with SOX9. S-nitrosylation at Cys-619 within adherens junctions promotes VEGF-induced, NO-dependent endothelial cell permeability by disrupting interaction with E-cadherin, thus mediating disassembly adherens junctions. O-glycosylation at Ser-23 decreases nuclear localization and transcriptional activity, and increases localization to the plasma membrane and interaction with E-cadherin CDH1. Deacetylated at Lys-49 by SIRT1.
Function Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Involved in the regulation of cell adhesion, as component of an E-cadherin:catenin adhesion complex. Acts as a negative regulator of centrosome cohesion. Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization. Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2. Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML. Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle. Involved in chondrocyte differentiation via interaction with SOX9: SOX9-binding competes with the binding sites of TCF/LEF within CTNNB1, thereby inhibiting the Wnt signaling.
Protein Name Catenin Beta-1
Beta-Catenin
Database Links Reactome: R-HSA-195253
Reactome: R-HSA-196299
Reactome: R-HSA-201681
Reactome: R-HSA-201722
Reactome: R-HSA-3134973
Reactome: R-HSA-351906
Reactome: R-HSA-3769402
Reactome: R-HSA-381771
Reactome: R-HSA-4086398
Reactome: R-HSA-418990
Reactome: R-HSA-4411364
Reactome: R-HSA-4641262
Reactome: R-HSA-5218920
Reactome: R-HSA-525793
Reactome: R-HSA-5339716
Reactome: R-HSA-5358747
Reactome: R-HSA-5358749
Reactome: R-HSA-5358751
Reactome: R-HSA-5358752
Reactome: R-HSA-5626467
Reactome: R-HSA-8853884
Reactome: R-HSA-8876493
Reactome: R-HSA-8951430
Cellular Localisation Cytoplasm
Nucleus
Cytoskeleton
Cell Junction
Adherens Junction
Cell Membrane
Microtubule Organizing Center
Centrosome
Spindle Pole
Synapse
Cilium Basal Body
Colocalized With Rapgef2 And Tjp1 At Cell-Cell Contacts
Cytoplasmic When It Is Unstabilized (High Level Of Phosphorylation) Or Bound To Cdh1
Translocates To The Nucleus When It Is Stabilized (Low Level Of Phosphorylation)
Interaction With Glis2 And Muc1 Promotes Nuclear Translocation
Interaction With Emd Inhibits Nuclear Localization
The Majority Of Beta-Catenin Is Localized To The Cell Membrane
In Interphase
Colocalizes With Crocc Between Cep250 Puncta At The Proximal End Of Centrioles
And This Localization Is Dependent On Crocc And Cep250
In Mitosis
When Nek2 Activity Increases
It Localizes To Centrosomes At Spindle Poles Independent Of Crocc
Colocalizes With Cdk5 In The Cell-Cell Contacts And Plasma Membrane Of Undifferentiated And Differentiated Neuroblastoma Cells
Interaction With Fam53b Promotes Translocation To The Nucleus
Alternative Antibody Names Anti-Catenin Beta-1 antibody
Anti-Beta-Catenin antibody
Anti-CTNNB1 antibody
Anti-CTNNB antibody
Anti-OK antibody
Anti-SW-cl.35 antibody
Anti-PRO2286 antibody

Information sourced from Uniprot.org

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