| Host: | Mouse |
| Applications: | IHC/WB |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-Podoplanin (23-100 aa) is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality : | Monoclonal |
| Clone ID : | ABT513 |
| Conjugation: | Unconjugated |
| Isotype: | IgG1k |
| Formulation: | Liquid in PBS pH7.2, 0.03% Proclin 300, with stabilizing protein. |
| Purification: | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Dilution Range: | IHC-P 1:200-400 WB 1:200-1000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PDPN |
| Gene ID: | 10630 |
| Uniprot ID: | PDPN_HUMAN |
| Immunogen Region: | 23-100 aa |
| Specificity: | The antibody can specifically recognize human Podoplanin protein. In western blotting of U2O2 cell lysate, the antibody can label a 36 kDa band corresponding to Podoplanin. |
| Immunogen: | Synthesized peptide derived from the human Podoplanin at the amino acid range 23-100 |
| Post Translational Modifications | Extensively O-glycosylated. Contains sialic acid residues. O-glycosylation is necessary for platelet aggregation activity. Disialylated at Thr-52.sialic acid is critical for platelet-aggregating activity and for CLEC1B interaction. The N-terminus is blocked. Cleaved by a metalloprotease within its extracellular (EC) domain, generating a membrane-bound C-terminal fragment (PCTF33) and an extracellular fragment. The resulting membrane-bound C-terminal fragment (PCTF33) is further processed between Val-150 and Val-151 by PSEN1/gamma-secretase generating the intracellular domain of podoplanin (PICD). |
| Function | Mediates effects on cell migration and adhesion through its different partners. During development plays a role in blood and lymphatic vessels separation by binding CLEC1B, triggering CLEC1B activation in platelets and leading to platelet activation and/or aggregation. Interaction with CD9, on the contrary, attenuates platelet aggregation induced by PDPN. Through MSN or EZR interaction promotes epithelial-mesenchymal transition (EMT) leading to ERZ phosphorylation and triggering RHOA activation leading to cell migration increase and invasiveness. Interaction with CD44 promotes directional cell migration in epithelial and tumor cells. In lymph nodes (LNs), controls fibroblastic reticular cells (FRCs) adhesion to the extracellular matrix (ECM) and contraction of the actomyosin by maintaining ERM proteins (EZR.MSN and RDX) and MYL9 activation through association with unknown transmembrane proteins. Engagement of CLEC1B by PDPN promotes FRCs relaxation by blocking lateral membrane interactions leading to reduction of ERM proteins (EZR.MSN and RDX) and MYL9 activation. Through binding with LGALS8 may participate in connection of the lymphatic endothelium to the surrounding extracellular matrix. In keratinocytes, induces changes in cell morphology showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Controls invadopodia stability and maturation leading to efficient degradation of the extracellular matrix (ECM) in tumor cells through modulation of RHOC activity in order to activate ROCK1/ROCK2 and LIMK1/LIMK2 and inactivation of CFL1. Required for normal lung cell proliferation and alveolus formation at birth. Does not function as a water channel or as a regulator of aquaporin-type water channels. Does not have any effect on folic acid or amino acid transport. |
| Protein Name | Podoplanin Aggrus Glycoprotein 36 Gp36 Pa2.26 Antigen T1-Alpha T1a Cleaved Into - 29kda Cytosolic Podoplanin Intracellular Domain Picd |
| Database Links | Reactome: R-HSA-114604 Reactome: R-HSA-9827857 |
| Cellular Localisation | Podoplanin: Membrane Single-Pass Type I Membrane Protein Cell Projection Lamellipodium Membrane Filopodium Membrane Microvillus Membrane Ruffle Membrane Membrane Raft Apical Cell Membrane Basolateral Cell Membrane Invadopodium Localized To Actin-Rich Microvilli And Plasma Membrane Projections Such As Filopodia Lamellipodia And Ruffles Association To The Lipid Rafts Is Required For Pdpn-Induced Epithelial To Mesenchymal Transition (Emt) Colocalizes With Cd9 In Tetraspanin Microdomains Localized At Invadopodium Adhesion Rings In Tumor Cell Association To The Lipid Rafts Is Essential For Pdpn Recruitment To Invadopodia And Ecm Degradation 29kda Cytosolic Podoplanin Intracellular Domain: Cytoplasm Cytosol |
| Alternative Antibody Names | Anti-Podoplanin antibody Anti-Aggrus antibody Anti-Glycoprotein 36 antibody Anti-Gp36 antibody Anti-Pa2.26 Antigen antibody Anti-T1-Alpha antibody Anti-T1a Cleaved Into - 29kda Cytosolic Podoplanin Intracellular Domain antibody Anti-Picd antibody Anti-PDPN antibody Anti-GP36 antibody Anti-PSEC0003 antibody Anti-PSEC0025 antibody |
Information sourced from Uniprot.org